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- W3029804547 abstract "目的 研制国内用于HBsAg检测的血清标准物质.方法 用HBV血清学标志物、抗-HCV、抗-HIV均为阴性的献血员血浆,混合均匀,将HBsAg阳性血浆稀释至含量约40 IU/ml,然后进行真空冷冻干燥.检测方法采用电化学发光(ECLA)、化学发光免疫试验(CIA)和ELISA三种方法与国际标准(NIBSC编号:00/588)同时检测,将结果进行比对.结果 该标准物质定值为:(34.2±4.8)IU/ml.其稳定性实验表明室温(20～25 ℃,相对湿度20%～50%)可稳定4周;高温高湿度(37℃,相对湿度60%～80%)条件下,可稳定2周;在冰箱冷藏(2～8 ℃)保存,可稳定1年;-20 ℃可稳定1年以上.均一性检测结果:所采用的检测方法表明均匀性良好,无明显差异.结论 该制备物可以作为HBsAg免疫测定的血清标准物质。" @default.
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- W3029804547 date "2007-08-11" @default.
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- W3029804547 title "Establishment of the first reference materials for hepatitis B surface antigen immunoassay" @default.
- W3029804547 doi "https://doi.org/10.3760/j.issn:1009-9158.2007.08.005" @default.
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