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- W3029830014 abstract "ObjectiveTo establish a method for the simultaneous content determination of quercetin, kaempferol and pinocembrin in Penthorum Chinense Pursh.MethodsThe HPLC analysis was carried out on Alltima C18 (250 mm × 4.6 mm, 5 μm) with a mixture of methanol-0.2% phosphate (45:55) as the mobile phase. The determination wavelength was set at 270 nm with the flow rate of 1.0 ml/min. The column temperature was set at 30 ℃.ResultsThe quercetin , kaempferol and pinocembrin showed good linearity in the range of 0.316-10.120 μg (r=0.999 9), 0.012-0.397 μg (r=0.999 6) and 0.063-2.016 μg (r=0.999 8) respectively. The average recovery rates of quercetin, kaempferol and pinocembrin were 99.34% (RSD=1.51%), 99.76% (RSD=1.96%) and 98.34% (RSD=1.56%) respectively.ConclusionsThe method is accurate and sensitive, which can be used for the content determination of quercetin, kaempferol and pinocembrin in Penthorum Chinense Pursh.Key words: Penthorum Chinense pursh; Quercetin; Kaempferol; Pinocembrin; High performance liquid chromatography" @default.
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- W3029830014 date "2019-08-30" @default.
- W3029830014 modified "2023-09-24" @default.
- W3029830014 title "Simultaneous determination of three compounds in penthorum Chinense pursh by HPLC" @default.
- W3029830014 doi "https://doi.org/10.3760/cma.j.issn.1673-4246.2019.08.016" @default.
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