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- W3029849043 abstract "Objective To explore the performance and application of Ficoll-Sephadex G-100 miacrocolumn technique for blood group serology.Methods Ficoll-Sephadex G-100 microcolumn was made of Sephadex G-100 by joining Ficoll liquid separation.200 specimens of ABO and Rh (D) blood type were identified by Ficoll-Sephadex G-100 microcolumn and BioVue.The difference between the two methods was compared.Six specimens of cold agglutinin syndrome in different titers were detected by BioVue incubating at 37 ℃ and 45 ℃ respectively,and by Ficoll-Sephadex G-100 microcolumn incubating at 45 ℃.26 hemolysis samples were selected for ABO and Rh (D) blood group identification by Ficoll-Sephadex G-100 microcolumn,BioVue,and Sephadex G-100 microcolumn respectively.Results The results of the 200 samples tested by Home-made blood group cards were consistent with those by BioVue and Sephadex G-100 microcolumn.The mean of anti-D antibody titers in the Home-made cards was 1: 256,which was consistent with that in BioVue.Home-made blood group cards were significant better than test tube method (P ＜ 0.05).The results of 6 cold agglutination samples were all correct for Home-made blood group cards,but 3 and 2 incorrect results incubated by BioVue at 37℃ and 45℃,respectively.The results of 26 hemolysis samples were all correct for Home-made blood group cards,6 and 10 incorrect results for BioVue Sephadex G-100 microcolumn.Conclusions Ficoll-Sephadex G-100 microcolumn has nice coincidence,sensitivity and repetitiveness and is a cheap and easy method.It is suitable for blood group typing.Key words: Ficoll liquid separation; Sephadex G-100 microcolumn; Blood group serological detecting; Cold agglutination; Hemolysis" @default.
- W3029849043 created "2020-06-05" @default.
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- W3029849043 date "2014-07-15" @default.
- W3029849043 modified "2023-09-26" @default.
- W3029849043 title "Development of Ficoll-Sephadex G-100 microcolumn technique for blood group serological detection" @default.
- W3029849043 doi "https://doi.org/10.3760/cma.j.issn.1007-1245.2014.14.046" @default.
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