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- W3029879503 abstract "ObjectiveTo identify micro RNA-122(miR-122)differentially expressed in pterygium and investigate the potential role of miR-122 in the pathogenesis of pterygium.MethodsQuantitative RT-PCR was used toidentifythe expression of miR-122 in pterygium tissue. The expression of CREB1, a predicted target of miR-122, and its correlation with the expression of miR-122, were detected in both pterygium and normal conjunctiva. The pterygium epithelial cells were isolated, cultured and then transfected with miR-122 mimic or miR-122 inhibitor respectively, to change miR-122 levels. The regulation of CREB1 expression by miR-122 was examined with luciferase activity assay, RT-PCR and Western blot. We also tested the protein levels of P53 and P21 by Western blot. The effect of the miR-122 on the apoptosis of cultured pterygium epithelial cells was investigated with AO/EB staining and caspase activity assay.ResultsThe expression of CREB1was significantly increased, and it is inversely related to the expression of miR-122 in pterygium, especially in the superficial layer of epithelium(P=0.031). In cultured pterygium epithelial cells, miR-122 could specifically bind to CREB1 3′UTR and negatively regulatethe expression of CREB1(P=0.033). Suppression of miR-122 could reduce apoptosis and caspase activity, and this effect was abolished by inhibition of the expression of CREB1 with specific siRNA(P=0.028, 0.029).ConclusionThe decreased expression of miR-122 in pterygium possibly results in abnormal cell apoptosis via its regulation of the expression of CREB1, and subsequently contributes to the development of pterygium.Key words: Pterygium; Apoptosis; miR-122; CREB1" @default.
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- W3029879503 date "2019-12-15" @default.
- W3029879503 modified "2023-09-23" @default.
- W3029879503 title "Regulation of apoptosis by miR-122 in pterygium via targeting CERB1 gene expression" @default.
- W3029879503 doi "https://doi.org/10.3760/cma.j.issn.1673-4386.2019.06.001" @default.
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