Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030038923> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3030038923 endingPage "211" @default.
- W3030038923 startingPage "207" @default.
- W3030038923 abstract "Objective To explore the feasibility of co-expressing recombinant baculovirus vector with dual promoters as dual-gene delivery vector.Methods After enhanced green fluorescent protein (EGFP) and glial cell line-derived neurotrophic factor (GDNF) respectively under cytomegalovirus (CMV) promoter were cloned into the pFastBac Dual vector,the recombinant plasmid pFastBac Dual-CMV-EGFP-CMV-GDNF was generated,and then transfected into HEK293T cells with Lipofectamine2000.Indirect immunofluorescence was applied to examining the co-expression of EGFP and GDNF in transfected 293T cells.Bac Dual-CMV-EGFP-CMV-GDNF was produced according to Bac-to-Bac Baculovirus Expression System manual,and used to transduce Hela cells at suitable MOI.Then indirect immunofluorescence and western blot analysis were used for detecting the existence of target proteins in Hela cells.Results Plasmid pFastBac DuaI-CMV-EGFP-CMV-GDNF and Bac Dual-CMV-EGFP-CMV-GDNF were successfully generated.EGFP and GDNF were co-expressed in one single 293T cell or Hela cell.Conclusions The pFastBac Dual vector was proved to be an effective dual-gene delivery vector,which supplied a new viral vector for multi-gene therapy.Key words: Baculovirus ; Dual-promoter; Co-expression; Dual-gene delivery vector; Multi-gene therapy" @default.
- W3030038923 created "2020-06-05" @default.
- W3030038923 creator A5009316585 @default.
- W3030038923 creator A5034545168 @default.
- W3030038923 creator A5036658104 @default.
- W3030038923 creator A5042174545 @default.
- W3030038923 creator A5047406489 @default.
- W3030038923 creator A5088685174 @default.
- W3030038923 creator A5090702709 @default.
- W3030038923 date "2013-10-25" @default.
- W3030038923 modified "2023-09-23" @default.
- W3030038923 title "Construction and identification of co-expressing recombinant baculovirus vector with dual promoters" @default.
- W3030038923 doi "https://doi.org/10.3760/cma.j.issn.1673-4092.2013.05.004" @default.
- W3030038923 hasPublicationYear "2013" @default.
- W3030038923 type Work @default.
- W3030038923 sameAs 3030038923 @default.
- W3030038923 citedByCount "0" @default.
- W3030038923 crossrefType "journal-article" @default.
- W3030038923 hasAuthorship W3030038923A5009316585 @default.
- W3030038923 hasAuthorship W3030038923A5034545168 @default.
- W3030038923 hasAuthorship W3030038923A5036658104 @default.
- W3030038923 hasAuthorship W3030038923A5042174545 @default.
- W3030038923 hasAuthorship W3030038923A5047406489 @default.
- W3030038923 hasAuthorship W3030038923A5088685174 @default.
- W3030038923 hasAuthorship W3030038923A5090702709 @default.
- W3030038923 hasConcept C101762097 @default.
- W3030038923 hasConcept C104317684 @default.
- W3030038923 hasConcept C111599444 @default.
- W3030038923 hasConcept C135983454 @default.
- W3030038923 hasConcept C142613039 @default.
- W3030038923 hasConcept C150194340 @default.
- W3030038923 hasConcept C153911025 @default.
- W3030038923 hasConcept C159047783 @default.
- W3030038923 hasConcept C160539049 @default.
- W3030038923 hasConcept C170493617 @default.
- W3030038923 hasConcept C174510640 @default.
- W3030038923 hasConcept C185856081 @default.
- W3030038923 hasConcept C22744801 @default.
- W3030038923 hasConcept C2777366897 @default.
- W3030038923 hasConcept C32470452 @default.
- W3030038923 hasConcept C33161422 @default.
- W3030038923 hasConcept C40767141 @default.
- W3030038923 hasConcept C54009773 @default.
- W3030038923 hasConcept C54355233 @default.
- W3030038923 hasConcept C81885089 @default.
- W3030038923 hasConcept C86803240 @default.
- W3030038923 hasConcept C92087593 @default.
- W3030038923 hasConceptScore W3030038923C101762097 @default.
- W3030038923 hasConceptScore W3030038923C104317684 @default.
- W3030038923 hasConceptScore W3030038923C111599444 @default.
- W3030038923 hasConceptScore W3030038923C135983454 @default.
- W3030038923 hasConceptScore W3030038923C142613039 @default.
- W3030038923 hasConceptScore W3030038923C150194340 @default.
- W3030038923 hasConceptScore W3030038923C153911025 @default.
- W3030038923 hasConceptScore W3030038923C159047783 @default.
- W3030038923 hasConceptScore W3030038923C160539049 @default.
- W3030038923 hasConceptScore W3030038923C170493617 @default.
- W3030038923 hasConceptScore W3030038923C174510640 @default.
- W3030038923 hasConceptScore W3030038923C185856081 @default.
- W3030038923 hasConceptScore W3030038923C22744801 @default.
- W3030038923 hasConceptScore W3030038923C2777366897 @default.
- W3030038923 hasConceptScore W3030038923C32470452 @default.
- W3030038923 hasConceptScore W3030038923C33161422 @default.
- W3030038923 hasConceptScore W3030038923C40767141 @default.
- W3030038923 hasConceptScore W3030038923C54009773 @default.
- W3030038923 hasConceptScore W3030038923C54355233 @default.
- W3030038923 hasConceptScore W3030038923C81885089 @default.
- W3030038923 hasConceptScore W3030038923C86803240 @default.
- W3030038923 hasConceptScore W3030038923C92087593 @default.
- W3030038923 hasIssue "05" @default.
- W3030038923 hasLocation W30300389231 @default.
- W3030038923 hasOpenAccess W3030038923 @default.
- W3030038923 hasPrimaryLocation W30300389231 @default.
- W3030038923 hasRelatedWork W2021897533 @default.
- W3030038923 hasRelatedWork W2043913008 @default.
- W3030038923 hasRelatedWork W2046035063 @default.
- W3030038923 hasRelatedWork W2107497648 @default.
- W3030038923 hasRelatedWork W2349293775 @default.
- W3030038923 hasRelatedWork W2349415525 @default.
- W3030038923 hasRelatedWork W2352352426 @default.
- W3030038923 hasRelatedWork W2353286559 @default.
- W3030038923 hasRelatedWork W2360372382 @default.
- W3030038923 hasRelatedWork W2361921620 @default.
- W3030038923 hasRelatedWork W2364785914 @default.
- W3030038923 hasRelatedWork W2372878785 @default.
- W3030038923 hasRelatedWork W2374428988 @default.
- W3030038923 hasRelatedWork W2381116097 @default.
- W3030038923 hasRelatedWork W2392201987 @default.
- W3030038923 hasRelatedWork W2412206585 @default.
- W3030038923 hasRelatedWork W2414579919 @default.
- W3030038923 hasRelatedWork W3030814607 @default.
- W3030038923 hasRelatedWork W650843890 @default.
- W3030038923 hasRelatedWork W2842866484 @default.
- W3030038923 hasVolume "20" @default.
- W3030038923 isParatext "false" @default.
- W3030038923 isRetracted "false" @default.
- W3030038923 magId "3030038923" @default.
- W3030038923 workType "article" @default.