FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030041186> ?p ?o ?g. }

• W3030041186 endingPage "1103" @default.
• W3030041186 startingPage "1101" @default.
• W3030041186 abstract "ObjectiveTo explore the influence of Rapamycin(RAPA) on apoptosis of acute lymphoblastic leukemia EU-4 cells induced by Methotrexate(MTX).MethodsEU-4 cells were pretreated 0.5 h with 5 μg/L, 10 μg/L, 25 μg/L, 50 μg/L and 100 μg/L RAPA.RAPA untreated group was set up as control group.The cells were collected and the expression of LC-3 was assayed by using Western blot.The apoptosis of EU-4 cells was detected by using flow cytometry(FCM). The effects of different concentrations of RAPA pretreatment on autophagy and apoptosis of EU-4 cells were observed, and the pretreatment concentration of RAPA was determined.EU-4 cells were divided into RAPA pretreatment group and untreated group.The cells were treated with 0.05 μmol/L, 0.10 μmol/L and 0.20 μmol/L MTX for 24 h, respectively.The apoptotic rate was detected by using FCM.Western blot was performed to test the expression of LC-3 protein, while the absorbance(A562) was measured by using microplate reader, and the protein concentration in the sample was calculated according to the standard curve.ResultsThe apoptotic rates of EU-4 cells in the control group and the different concentrations of RAPA pretreatment group showed that the control group, 5 μg/L, 10 μg/L, 25 μg/L, 50 μg/L and 100 μg/L RAPA were (7.51±0.32)%, (7.33±0.41)%, (7.71±0.51)%, (7.63±0.38)%, (7.80±0.43)% and (16.66±0.87)%, respectively.The apoptotic rates of EU-4 cells in the 5 μg/L, 10 μg/L, 25 μg/L and 50 μg/L RAPA pretreatment group had no significant difference from those in the control group(t=0.427, -0.417, -0.297, -3.561, all P>0.05). The apoptotic rate of EU-4 cells in 100 μg/L RAPA pretreatment group was significantly higher than that in control group, and the difference was significant(t=-28.815, P<0.01). Combined with the results of Western blot and FCM, 50 μg/L was used as the pretreatment of RAPA.The apoptosis rates of EU-4 cells in RAPA pretreatment group were (50.23±2.11)%, (66.88±2.89)% and (73.11±2.67)% after treated with 0.05 μmol/L, 0.10 μmol/L and 0.20 μmol/L MTX for 24 h, respectively.The apoptotic rates of EU-4 cells in RAPA unpretreatment group were (22.53±1.67)%, (42.82±2.26)% and (53.22±1.93)% after treated with 0.05 μmol/L, 0.10 μmol/L and 0.20 μmol/L MTX for 24 h, respectively.The apoptotic rates of EU-4 cells in RAPA pretreatment group were significantly higher than those in untreated group in the same concentration of MTX treatment after 24 h, and the differences were significant(t=12.693, 66.148, 14.429; all P<0.01).ConclusionWith RAPA pretreatment, relative low dose MTX can induce a great deal of acute lymphoblastic leukemia EU-4 cells apoptosis.Key words: Acute lymphoblastic leukemia; Rapamycin; Methotrexate" @default.
• W3030041186 created "2020-06-05" @default.
• W3030041186 creator A5001552700 @default.
• W3030041186 creator A5012427092 @default.
• W3030041186 creator A5020302124 @default.
• W3030041186 creator A5032109840 @default.
• W3030041186 creator A5036582057 @default.
• W3030041186 creator A5054342493 @default.
• W3030041186 creator A5089834150 @default.
• W3030041186 date "2017-07-20" @default.
• W3030041186 modified "2023-09-27" @default.
• W3030041186 title "Influence of Rapamycin on apoptosis of acute lymphoblastic leukemia EU-4 cells induced by Methotrexate" @default.
• W3030041186 doi "https://doi.org/10.3760/cma.j.issn.2095-428x.2017.14.015" @default.
• W3030041186 hasPublicationYear "2017" @default.
• W3030041186 type Work @default.
• W3030041186 sameAs 3030041186 @default.
• W3030041186 citedByCount "0" @default.
• W3030041186 crossrefType "journal-article" @default.
• W3030041186 hasAuthorship W3030041186A5001552700 @default.
• W3030041186 hasAuthorship W3030041186A5012427092 @default.
• W3030041186 hasAuthorship W3030041186A5020302124 @default.
• W3030041186 hasAuthorship W3030041186A5032109840 @default.
• W3030041186 hasAuthorship W3030041186A5036582057 @default.
• W3030041186 hasAuthorship W3030041186A5054342493 @default.
• W3030041186 hasAuthorship W3030041186A5089834150 @default.
• W3030041186 hasConcept C104317684 @default.
• W3030041186 hasConcept C153911025 @default.
• W3030041186 hasConcept C185592680 @default.
• W3030041186 hasConcept C190283241 @default.
• W3030041186 hasConcept C203014093 @default.
• W3030041186 hasConcept C2776415932 @default.
• W3030041186 hasConcept C2778461978 @default.
• W3030041186 hasConcept C2781059491 @default.
• W3030041186 hasConcept C2909962599 @default.
• W3030041186 hasConcept C553184892 @default.
• W3030041186 hasConcept C55493867 @default.
• W3030041186 hasConcept C86803240 @default.
• W3030041186 hasConceptScore W3030041186C104317684 @default.
• W3030041186 hasConceptScore W3030041186C153911025 @default.
• W3030041186 hasConceptScore W3030041186C185592680 @default.
• W3030041186 hasConceptScore W3030041186C190283241 @default.
• W3030041186 hasConceptScore W3030041186C203014093 @default.
• W3030041186 hasConceptScore W3030041186C2776415932 @default.
• W3030041186 hasConceptScore W3030041186C2778461978 @default.
• W3030041186 hasConceptScore W3030041186C2781059491 @default.
• W3030041186 hasConceptScore W3030041186C2909962599 @default.
• W3030041186 hasConceptScore W3030041186C553184892 @default.
• W3030041186 hasConceptScore W3030041186C55493867 @default.
• W3030041186 hasConceptScore W3030041186C86803240 @default.
• W3030041186 hasIssue "14" @default.
• W3030041186 hasLocation W30300411861 @default.
• W3030041186 hasOpenAccess W3030041186 @default.
• W3030041186 hasPrimaryLocation W30300411861 @default.
• W3030041186 hasRelatedWork W2349686623 @default.
• W3030041186 hasRelatedWork W2356578462 @default.
• W3030041186 hasRelatedWork W2364133014 @default.
• W3030041186 hasRelatedWork W2368012933 @default.
• W3030041186 hasRelatedWork W2369627817 @default.
• W3030041186 hasRelatedWork W2374568026 @default.
• W3030041186 hasRelatedWork W2376389804 @default.
• W3030041186 hasRelatedWork W2379638453 @default.
• W3030041186 hasRelatedWork W2382315494 @default.
• W3030041186 hasRelatedWork W2383214420 @default.
• W3030041186 hasRelatedWork W2391792022 @default.
• W3030041186 hasRelatedWork W2407829041 @default.
• W3030041186 hasRelatedWork W2410306527 @default.
• W3030041186 hasRelatedWork W3009359865 @default.
• W3030041186 hasRelatedWork W3031065899 @default.
• W3030041186 hasRelatedWork W3031480993 @default.
• W3030041186 hasRelatedWork W3031739751 @default.
• W3030041186 hasRelatedWork W3032555388 @default.
• W3030041186 hasRelatedWork W3032829687 @default.
• W3030041186 hasRelatedWork W3092602616 @default.
• W3030041186 hasVolume "32" @default.
• W3030041186 isParatext "false" @default.
• W3030041186 isRetracted "false" @default.
• W3030041186 magId "3030041186" @default.
• W3030041186 workType "article" @default.