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- W3030067612 abstract "Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.Key words: Carcinoma, hepatocellular; HBVE; Alpha-fetoproteins; Promoter regions (genetics); Interleukin-2; Targeting" @default.
- W3030067612 created "2020-06-05" @default.
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- W3030067612 date "2014-01-28" @default.
- W3030067612 modified "2023-09-28" @default.
- W3030067612 title "The targeting expression of IL-2 gene driven by alpha fetoprotein promoter and hepatitis B virus envelope in hepatic carcinoma cell" @default.
- W3030067612 doi "https://doi.org/10.3760/cma.j.issn.1006-9801.2014.01.006" @default.
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