FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030097851> ?p ?o ?g. }

• W3030097851 endingPage "1110" @default.
• W3030097851 startingPage "1107" @default.
• W3030097851 abstract "ObjectiveTo observe the CD105- biotin- avidin promoting synovium- derived mesenchymal stem cells(SMSCs)adhesion and selecting CD105- positive cells in the cartilage tissue engineering, and evaluate its effects on cartilage formation.MethodsSMSCs were obtained by enzyme digestion.Flow cytometry was used to detect markers on cell surface, including CD29, CD34, CD44, CD45, CD90 and CD105.With divided 3 groups(A:untreated+ cells; B:avidin+ biotin- cells; C:avidin+ CD105- biotin - cells), adhesion cell number was counted by absorbance and random field count at different time point.CD105- positive cells were tested through immunofluorescence. After 3 weeks chondrogenic induction, hematoxylin- eosin(HE), alcian blue staining and immunofluorescence were used to assess chondrogenesis.ResultsSMSCs showed star or spindle shape with adherent growth. The expression of CD29, CD44 and CD90 was positive, while CD34 and CD45 negative.The positive rate of CD105 was about 42%.Compared with Group A, Group B and C significantly increased cells adhesion at 1 and 2 h(P< 0.05). Although Group B had more cells than C, there was no statistical difference.Immunofluorescence showed CD105- positive cells in Group C were significantly higher than that in Group B and A.After chondrogenic induction, while proteoglycan and typeⅡcollagen were observed in all groups, there was most cartilage specific extracellular matrix in Group C.Con-clusionCD105- biotin- avidin system could not only enhance cells adhesion, but also select CD105 - positive cells, which may promote chondrogenesis better.Key words: CD105; Biotin-avidin; Synovium-derived mesenchymal stem cells; Cartilage tissue engineering" @default.
• W3030097851 created "2020-06-05" @default.
• W3030097851 creator A5018821173 @default.
• W3030097851 creator A5043296622 @default.
• W3030097851 creator A5052858850 @default.
• W3030097851 creator A5063253432 @default.
• W3030097851 creator A5088087415 @default.
• W3030097851 date "2015-05-08" @default.
• W3030097851 modified "2023-09-27" @default.
• W3030097851 title "CD105- biotin- avidin promoting synovium- derived mesenchymal stem cells adhesion and selection" @default.
• W3030097851 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2015.05.054" @default.
• W3030097851 hasPublicationYear "2015" @default.
• W3030097851 type Work @default.
• W3030097851 sameAs 3030097851 @default.
• W3030097851 citedByCount "0" @default.
• W3030097851 crossrefType "journal-article" @default.
• W3030097851 hasAuthorship W3030097851A5018821173 @default.
• W3030097851 hasAuthorship W3030097851A5043296622 @default.
• W3030097851 hasAuthorship W3030097851A5052858850 @default.
• W3030097851 hasAuthorship W3030097851A5063253432 @default.
• W3030097851 hasAuthorship W3030097851A5088087415 @default.
• W3030097851 hasConcept C10205521 @default.
• W3030097851 hasConcept C105702510 @default.
• W3030097851 hasConcept C116716535 @default.
• W3030097851 hasConcept C142724271 @default.
• W3030097851 hasConcept C1491633281 @default.
• W3030097851 hasConcept C153911025 @default.
• W3030097851 hasConcept C159654299 @default.
• W3030097851 hasConcept C185592680 @default.
• W3030097851 hasConcept C189165786 @default.
• W3030097851 hasConcept C197534660 @default.
• W3030097851 hasConcept C198826908 @default.
• W3030097851 hasConcept C203014093 @default.
• W3030097851 hasConcept C204787440 @default.
• W3030097851 hasConcept C2776164576 @default.
• W3030097851 hasConcept C2778236600 @default.
• W3030097851 hasConcept C2780550940 @default.
• W3030097851 hasConcept C2780932548 @default.
• W3030097851 hasConcept C28328180 @default.
• W3030097851 hasConcept C3020332539 @default.
• W3030097851 hasConcept C35496256 @default.
• W3030097851 hasConcept C55493867 @default.
• W3030097851 hasConcept C6061869 @default.
• W3030097851 hasConcept C71924100 @default.
• W3030097851 hasConcept C86803240 @default.
• W3030097851 hasConcept C95444343 @default.
• W3030097851 hasConceptScore W3030097851C10205521 @default.
• W3030097851 hasConceptScore W3030097851C105702510 @default.
• W3030097851 hasConceptScore W3030097851C116716535 @default.
• W3030097851 hasConceptScore W3030097851C142724271 @default.
• W3030097851 hasConceptScore W3030097851C1491633281 @default.
• W3030097851 hasConceptScore W3030097851C153911025 @default.
• W3030097851 hasConceptScore W3030097851C159654299 @default.
• W3030097851 hasConceptScore W3030097851C185592680 @default.
• W3030097851 hasConceptScore W3030097851C189165786 @default.
• W3030097851 hasConceptScore W3030097851C197534660 @default.
• W3030097851 hasConceptScore W3030097851C198826908 @default.
• W3030097851 hasConceptScore W3030097851C203014093 @default.
• W3030097851 hasConceptScore W3030097851C204787440 @default.
• W3030097851 hasConceptScore W3030097851C2776164576 @default.
• W3030097851 hasConceptScore W3030097851C2778236600 @default.
• W3030097851 hasConceptScore W3030097851C2780550940 @default.
• W3030097851 hasConceptScore W3030097851C2780932548 @default.
• W3030097851 hasConceptScore W3030097851C28328180 @default.
• W3030097851 hasConceptScore W3030097851C3020332539 @default.
• W3030097851 hasConceptScore W3030097851C35496256 @default.
• W3030097851 hasConceptScore W3030097851C55493867 @default.
• W3030097851 hasConceptScore W3030097851C6061869 @default.
• W3030097851 hasConceptScore W3030097851C71924100 @default.
• W3030097851 hasConceptScore W3030097851C86803240 @default.
• W3030097851 hasConceptScore W3030097851C95444343 @default.
• W3030097851 hasIssue "05" @default.
• W3030097851 hasLocation W30300978511 @default.
• W3030097851 hasOpenAccess W3030097851 @default.
• W3030097851 hasPrimaryLocation W30300978511 @default.
• W3030097851 hasRelatedWork W2022174393 @default.
• W3030097851 hasRelatedWork W2351552098 @default.
• W3030097851 hasRelatedWork W2354087386 @default.
• W3030097851 hasRelatedWork W2360522176 @default.
• W3030097851 hasRelatedWork W2366020751 @default.
• W3030097851 hasRelatedWork W2367581414 @default.
• W3030097851 hasRelatedWork W2374289587 @default.
• W3030097851 hasRelatedWork W2381207352 @default.
• W3030097851 hasRelatedWork W2381865402 @default.
• W3030097851 hasRelatedWork W2384531754 @default.
• W3030097851 hasRelatedWork W2384643064 @default.
• W3030097851 hasRelatedWork W2398588396 @default.
• W3030097851 hasRelatedWork W2412793131 @default.
• W3030097851 hasRelatedWork W2483197751 @default.
• W3030097851 hasRelatedWork W2577738690 @default.
• W3030097851 hasRelatedWork W2783705414 @default.
• W3030097851 hasRelatedWork W3017221757 @default.
• W3030097851 hasRelatedWork W3029224797 @default.
• W3030097851 hasRelatedWork W3031691698 @default.
• W3030097851 hasRelatedWork W2558774102 @default.
• W3030097851 hasVolume "32" @default.
• W3030097851 isParatext "false" @default.
• W3030097851 isRetracted "false" @default.

• next