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- W3030143385 abstract "ObjectiveTo study the impact of downregulating the negative costimulation molecule B7-H1 on biological behaviors in esophageal cancer cell line Eca-109.MethodsDown-regulation of B7-H1 was performed by using RNAi method in the human esophageal cancer cell line Eca-109. The expression level of B7-H1 was confirmed by using flow cytometry and Western blotting. The cell counting kit-8 (CCK-8) assay, scratch assay and Transwell assay were used to investigate the contribution of B7-H1 to biological behaviors of esophageal cancer cells.ResultsWe successfully constructed stably reduced expression of B7-H1 in human esophageal cancer cell line by using RNAi named Eca109-B7-H1-small interfering RNA (siRNA) and the control group Eca109-NC. The CCK-8 cell proliferation experiment showed that on the day 6, 7 and 8, the proliferation rate in Eca109-B7-H1-siRNA group (0.61±0.03, 0.78±0.04, and 0.89±0.04) was significantly lower than in the control group (0.80±0.01, 1.00±0.04, and 1.07±0.09; P 0.05). The scratch assay results revealed that the cell migration ability in Eca109-B7-H1-siRNA group [(126.00±22.63) μm was significantly reduced as compared with the control group [(339.00±22.11) μm] at 24 h (P 0.05). Transwell invasion assay results demonstrated that at 24 h after the cells were seeded, the number of invasive cells in Eca109-B7-H1-siRNA group [(20.60±4.61) cells] was singnificantly less than in the control group [(60.40±12.50) cells, P<0.01].ConclusionDown-regulation of B7-H1 in esophageal cancer cell line Eca-109 could significantly inhibit the cell proliferation, migration and invasion, suggesting that the abnormal expression of B7-H1 in esophageal carcinoma cells could be involved in regulating the tumor biological behaviors and contribute to the tumor progression.Key words: B7-H1; Esophageal cancer; Eca-109; RNA interference" @default.
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- W3030143385 date "2016-12-08" @default.
- W3030143385 modified "2023-09-27" @default.
- W3030143385 title "B7-H1 expression and its contribution to the regulation of biological function in human esophageal cancer cell line" @default.
- W3030143385 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2016.12.029" @default.
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