Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030198712> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W3030198712 endingPage "844" @default.
- W3030198712 startingPage "841" @default.
- W3030198712 abstract "Objective To construct a universal influenza virus vaccine Baemid containing M2 extracellular domain(M2e) of influenza virus which conjugated human papillomavirus(HPV)16 L1 as a vector and expressed the protein in insect baeulovirus system. Methods HPV16 L1 gene was amplified by PCR with the primers contained M2e gene. The conjugated genes were inserted into pFast HTA vector to recombine in DHI0Bac. The recombinant baemid was transfected into sf9 insect cells by liposome to produce baculovirus contained M2e-HPVI6 L1. Protein was determined by SDS-PAGE, immunofluorescence and electronmicroseope. Results The universal influenza virus vaccine Bacmid containing M2e of influenza virus which conjugated HPV16 L1 as a vector was successfully constructed. The recombinant protein wasexpressed in insect cells. Conclusions The recombinant protein was expressed in insect cells in the form of virus-like particles through the baculovirus system,which was a base of the universal influenza virus vaccine development.Key words: M2e of influenza A virus; Virus-like particle; Insect baculovirus system" @default.
- W3030198712 created "2020-06-05" @default.
- W3030198712 creator A5091701285 @default.
- W3030198712 date "2008-07-20" @default.
- W3030198712 modified "2023-09-23" @default.
- W3030198712 title "Research of universal influenza virus vaccine conjugated human papillomavirus 16 LI as a vector" @default.
- W3030198712 doi "https://doi.org/10.3760/cma.j.issn.1673-436x.2008.14.004" @default.
- W3030198712 hasPublicationYear "2008" @default.
- W3030198712 type Work @default.
- W3030198712 sameAs 3030198712 @default.
- W3030198712 citedByCount "0" @default.
- W3030198712 crossrefType "journal-article" @default.
- W3030198712 hasAuthorship W3030198712A5091701285 @default.
- W3030198712 hasConcept C103878061 @default.
- W3030198712 hasConcept C104317684 @default.
- W3030198712 hasConcept C159047783 @default.
- W3030198712 hasConcept C159654299 @default.
- W3030198712 hasConcept C2522874641 @default.
- W3030198712 hasConcept C2776387124 @default.
- W3030198712 hasConcept C2777773057 @default.
- W3030198712 hasConcept C2778236600 @default.
- W3030198712 hasConcept C40767141 @default.
- W3030198712 hasConcept C54009773 @default.
- W3030198712 hasConcept C54355233 @default.
- W3030198712 hasConcept C86803240 @default.
- W3030198712 hasConcept C92087593 @default.
- W3030198712 hasConceptScore W3030198712C103878061 @default.
- W3030198712 hasConceptScore W3030198712C104317684 @default.
- W3030198712 hasConceptScore W3030198712C159047783 @default.
- W3030198712 hasConceptScore W3030198712C159654299 @default.
- W3030198712 hasConceptScore W3030198712C2522874641 @default.
- W3030198712 hasConceptScore W3030198712C2776387124 @default.
- W3030198712 hasConceptScore W3030198712C2777773057 @default.
- W3030198712 hasConceptScore W3030198712C2778236600 @default.
- W3030198712 hasConceptScore W3030198712C40767141 @default.
- W3030198712 hasConceptScore W3030198712C54009773 @default.
- W3030198712 hasConceptScore W3030198712C54355233 @default.
- W3030198712 hasConceptScore W3030198712C86803240 @default.
- W3030198712 hasConceptScore W3030198712C92087593 @default.
- W3030198712 hasIssue "14" @default.
- W3030198712 hasLocation W30301987121 @default.
- W3030198712 hasOpenAccess W3030198712 @default.
- W3030198712 hasPrimaryLocation W30301987121 @default.
- W3030198712 hasRelatedWork W2059237302 @default.
- W3030198712 hasRelatedWork W2088911729 @default.
- W3030198712 hasRelatedWork W2222794528 @default.
- W3030198712 hasRelatedWork W2277079997 @default.
- W3030198712 hasRelatedWork W2379023457 @default.
- W3030198712 hasRelatedWork W2394247790 @default.
- W3030198712 hasRelatedWork W2765633725 @default.
- W3030198712 hasRelatedWork W2108595148 @default.
- W3030198712 hasRelatedWork W2817245783 @default.
- W3030198712 hasRelatedWork W2820171260 @default.
- W3030198712 hasRelatedWork W2822720732 @default.
- W3030198712 hasRelatedWork W2826521560 @default.
- W3030198712 hasRelatedWork W2842105243 @default.
- W3030198712 hasRelatedWork W2856148409 @default.
- W3030198712 hasRelatedWork W2869501724 @default.
- W3030198712 hasRelatedWork W2879397150 @default.
- W3030198712 hasRelatedWork W2916601339 @default.
- W3030198712 hasRelatedWork W2927072665 @default.
- W3030198712 hasRelatedWork W2958584085 @default.
- W3030198712 hasRelatedWork W3196245884 @default.
- W3030198712 hasVolume "28" @default.
- W3030198712 isParatext "false" @default.
- W3030198712 isRetracted "false" @default.
- W3030198712 magId "3030198712" @default.
- W3030198712 workType "article" @default.