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- W3030210880 abstract "ObjectiveTo investigate the effects of synuclein-γ (SNCG) gene silencing on the proliferation and apoptosis of glioma U87-MG cells.MethodsFive small hairpin RNA templates targeting SNCG and a negative control were synthesized and cloned into the lentiviral vector system and all the constructs were sequenced. Then the recombinant lentiviral vectors were used to infect U87-MG cells. The lentiviruses which can effectively inhibit protein expression levels of SNCG were selected by RT-PCR for further study. Colony formation and flow cytometry assay were used to investigate the effects of SNCG downregulation by RNA interference on the clony formation, proliferation, and apoptosis of U87-MG cells, respectively.ResultsThe lentiviral vectors carrying 5 shRNAs targeting the SNCG gene were successfully constructed, and SNCG siRNA3 and siRNA5 showed higher interfering efficiency than other vectors. In comparison with the group of negative control, SNCG siRNA3 and siRNA5 were observed to significantly inhibit SNCG expression at the mRNA levels (the relative mRNA levels: siRNA3 (0.17±0.01)%, siRNA5 (0.13±0.01)% vs (1.00±0.10)%, P<0.05). Also, SNCG suppression mediated by RNAi significantly inhibited the clone formation (colony number: siRNA3 (66±12), siRNA5 (1±1) vs (80±5), P<0.05), and the proliferation (ratio of cells in S phase: siRNA3 (41.2±0.7)%, siRNA5 (39.9±0.5)% vs (47.6±2.2)%, P<0.05), but promoted the apoptosis (cell apoptosis: siRNA3 (22.9±0.4)%, siRNA5 (28.6±0.9)% vs (1.1±0.1)%, P<0.01) of transfected U87-MG cells.ConclusionSNCG suppression at the mRNA level mediated by RNAi can inhibit the proliferation and the clony formation, but induce the apoptosis of glioma U87-MG cells in vitro, suggesting that SNCG suppression mediated by an RNAi strategy may become a novel approach for treating human gliomas.Key words: Glioma; Synuclein-γ; RNA interference; Cell proliferation; Cell apoptosis" @default.
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- W3030210880 date "2015-11-20" @default.
- W3030210880 modified "2023-09-28" @default.
- W3030210880 title "Synuclein-γ suppression mediated by RNA interference inhibits proliferation and promotes apoptosis of human glioma U87-MG cells" @default.
- W3030210880 doi "https://doi.org/10.3760/cma.j.issn.1674-6554.2015.11.001" @default.
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