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• W3030227293 abstract "ObjectiveTo investigate the effects of ch1D1, an anti-CD86 chimeric antibody, on autoreactive B lymphocytes isolated from patients with systemic lupus erythematosus (SLE).MethodsFlow cytometry analysis was performed to measure the expression of CD86 on the surface of B cells isolated from patients with SLE and to analyze the effects of ch1D1 on the activation of CD4+ T cells. The method of magnetic bead sorting was used to separate B cells, NK cells and CD4+ T cells from PBMC collected from healthy subjects and patients with SLE for subsequent experiments. Antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) that were mediated by ch1D1 were measured with LDH release assay. Effects of ch1D1 on the secretion of auto-antibodies and the proliferation of CD4+ T were detected by ELISA and 3H-thymidine (3H-TdR) incorporation assay, respectively.ResultsThe levels of CD80 (68.08±14.28 vs 46.10±12.14, n=24, P<0.000 1) and CD86 (44.72±14.90 vs 13.99±10.74, n=24, P<0.000 1) expressed on the surface of B cells isolated from patients with SLE were significantly higher than those from the healthy subjects, suggesting the abnormal activation of B cells. Compared with the negative control group and the murine monoclonal antibody 1D1, ch1D1 was more effective in mediating the ADCC and CDC responses (P=0.017 2, P=0.038 8). Activated T cells significantly enhanced the secretion of auto-antibodies by B cells isolated from patients with SLE. Compared with the negative control group, the enhanced secretion of auto-antibodies was significantly inhibited by treatment with ch1D1 (P=0.001 9). Moreover, ch1D1 significantly inhibited the proliferation and activation of CD4+ T cells induced in patients with SLE (P=0.002 4, P=0.049 5).Conclusionch1D1, the anti-CD86 chimeric antibody, could effectively mediate the ADCC and CDC responses against autoreactive B cells isolated from patients with SLE, inhibit the secretion of auto-antibodies and suppress the proliferation and activation of auto-reactive CD4+ T cells. It might be a potential immunotherapy agent for the treatment of SLE.Key words: CD86; Chimeric antibody; SLE; Autoreactive B cells; Autoantibody" @default.
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• W3030227293 date "2016-07-31" @default.
• W3030227293 modified "2023-09-24" @default.
• W3030227293 title "Effects of an anti-CD86 chimeric antibody (ch1D1) on autoreactive B lymphocytes isolated from patients with SLE" @default.
• W3030227293 doi "https://doi.org/10.3760/cma.j.issn.0254-5101.2016.07.001" @default.
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