Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030232634> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W3030232634 abstract "Abstract Introduction The elevated cancer risk associated with night shift work is poorly understood. To investigate whether circadian disruption may play a role, we assessed the circadian transcriptome and its association with hallmark cancer pathways, as well as sensitivity to endogenous and exogenous sources of DNA damage, after in-laboratory simulated shift work. Methods N=14 healthy humans (aged 22-34y; 10m, 4f) were exposed to a simulated night shift schedule (daytime sleep: 10:00-18:00) or a simulated day shift schedule (nighttime sleep: 22:00-06:00) for 3 days (n=7 in each condition). After the simulated shift schedule, subjects underwent a 24h constant routine protocol, during which blood was collected every 3h. Lymphocytes were extracted from the blood samples and subjected to transcriptome analysis using a NanoString multiplex assay. We evaluated 726 mRNA cancer hallmark targets (NanoString PanCancer Pathway Panel) and 17 circadian clock genes, with 18 arrhythmic internal controls. Gene expression was analyzed for circadian rhythmicity using mixed-effects cosinor analysis. Further, lymphocytes were investigated for DNA damage using an alkaline comet assay and immunofluorescence assessment of DNA damage response biomarkers BRCA1 and γH2AX. Lymphocytes collected at 07:30 and 19:30 were also exposed to ionizing radiation (2.5Gy) and DNA damage response assessments were repeated. Results Simulated night shift caused widespread disruption of circadian rhythmicity, as measured under constant routine, for core clock genes and the transcriptome of cancer hallmark pathways. The DNA repair pathway showed significant enrichment of rhythmic genes (p<0.05) after the simulated day shift schedule only. Following simulated night shift, lymphocytes showed induction of endogenous DNA damage, with extended tail in the comet assay (p<0.001), and higher percentage of lymphocytes with BRCA1 and γH2AX foci (p<0.01). Lymphocytes collected at 19:30 showed enhanced impact of ionizing radiation as indicated by increased prevalence of cells with BRCA1 and γH2AX foci (p<0.05). Conclusion Circadian dysregulation of DNA repair mechanisms and increased sensitivity to DNA damage following night shift work may increase genomic instability and precipitate elevated cancer risk in night shift workers. Support NIH grants ES022640 and CA227381, CDMRP award W81XWH-18-1-0100, and Pacific Northwest National Laboratory BRAVE investment under DOE contract DE-AC05-76RL01830." @default.
- W3030232634 created "2020-06-05" @default.
- W3030232634 creator A5000376180 @default.
- W3030232634 creator A5018289615 @default.
- W3030232634 creator A5020608405 @default.
- W3030232634 creator A5025675631 @default.
- W3030232634 creator A5036030045 @default.
- W3030232634 creator A5050158902 @default.
- W3030232634 creator A5057745514 @default.
- W3030232634 creator A5061514063 @default.
- W3030232634 creator A5062394853 @default.
- W3030232634 creator A5071330471 @default.
- W3030232634 date "2020-04-01" @default.
- W3030232634 modified "2023-09-27" @default.
- W3030232634 title "0025 Circadian Dysregulation of DNA Repair and Increased Endogenous and Exogenous Sensitivity to DNA Damage Precipitate Elevated Cancer Risk Associated with Night Shift Work" @default.
- W3030232634 doi "https://doi.org/10.1093/sleep/zsaa056.024" @default.
- W3030232634 hasPublicationYear "2020" @default.
- W3030232634 type Work @default.
- W3030232634 sameAs 3030232634 @default.
- W3030232634 citedByCount "0" @default.
- W3030232634 crossrefType "journal-article" @default.
- W3030232634 hasAuthorship W3030232634A5000376180 @default.
- W3030232634 hasAuthorship W3030232634A5018289615 @default.
- W3030232634 hasAuthorship W3030232634A5020608405 @default.
- W3030232634 hasAuthorship W3030232634A5025675631 @default.
- W3030232634 hasAuthorship W3030232634A5036030045 @default.
- W3030232634 hasAuthorship W3030232634A5050158902 @default.
- W3030232634 hasAuthorship W3030232634A5057745514 @default.
- W3030232634 hasAuthorship W3030232634A5061514063 @default.
- W3030232634 hasAuthorship W3030232634A5062394853 @default.
- W3030232634 hasAuthorship W3030232634A5071330471 @default.
- W3030232634 hasBestOaLocation W30302326341 @default.
- W3030232634 hasConcept C104317684 @default.
- W3030232634 hasConcept C121446783 @default.
- W3030232634 hasConcept C126322002 @default.
- W3030232634 hasConcept C134018914 @default.
- W3030232634 hasConcept C134935766 @default.
- W3030232634 hasConcept C143425029 @default.
- W3030232634 hasConcept C150194340 @default.
- W3030232634 hasConcept C162317418 @default.
- W3030232634 hasConcept C16613235 @default.
- W3030232634 hasConcept C169760540 @default.
- W3030232634 hasConcept C184738001 @default.
- W3030232634 hasConcept C2776176791 @default.
- W3030232634 hasConcept C2780358455 @default.
- W3030232634 hasConcept C38606739 @default.
- W3030232634 hasConcept C54355233 @default.
- W3030232634 hasConcept C552990157 @default.
- W3030232634 hasConcept C71924100 @default.
- W3030232634 hasConcept C86803240 @default.
- W3030232634 hasConceptScore W3030232634C104317684 @default.
- W3030232634 hasConceptScore W3030232634C121446783 @default.
- W3030232634 hasConceptScore W3030232634C126322002 @default.
- W3030232634 hasConceptScore W3030232634C134018914 @default.
- W3030232634 hasConceptScore W3030232634C134935766 @default.
- W3030232634 hasConceptScore W3030232634C143425029 @default.
- W3030232634 hasConceptScore W3030232634C150194340 @default.
- W3030232634 hasConceptScore W3030232634C162317418 @default.
- W3030232634 hasConceptScore W3030232634C16613235 @default.
- W3030232634 hasConceptScore W3030232634C169760540 @default.
- W3030232634 hasConceptScore W3030232634C184738001 @default.
- W3030232634 hasConceptScore W3030232634C2776176791 @default.
- W3030232634 hasConceptScore W3030232634C2780358455 @default.
- W3030232634 hasConceptScore W3030232634C38606739 @default.
- W3030232634 hasConceptScore W3030232634C54355233 @default.
- W3030232634 hasConceptScore W3030232634C552990157 @default.
- W3030232634 hasConceptScore W3030232634C71924100 @default.
- W3030232634 hasConceptScore W3030232634C86803240 @default.
- W3030232634 hasLocation W30302326341 @default.
- W3030232634 hasOpenAccess W3030232634 @default.
- W3030232634 hasPrimaryLocation W30302326341 @default.
- W3030232634 hasRelatedWork W1255473 @default.
- W3030232634 hasRelatedWork W14107102 @default.
- W3030232634 hasRelatedWork W1452304 @default.
- W3030232634 hasRelatedWork W2515022 @default.
- W3030232634 hasRelatedWork W4149045 @default.
- W3030232634 hasRelatedWork W5893096 @default.
- W3030232634 hasRelatedWork W6978838 @default.
- W3030232634 hasRelatedWork W7042538 @default.
- W3030232634 hasRelatedWork W7217652 @default.
- W3030232634 hasRelatedWork W8261484 @default.
- W3030232634 isParatext "false" @default.
- W3030232634 isRetracted "false" @default.
- W3030232634 magId "3030232634" @default.
- W3030232634 workType "article" @default.