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- W3030247948 abstract "ObjectiveTo compare the effects of islet-like cells originated from different generations of rat bone marrow-derived mesenchymal stem cells (BMSCs) with pancreatic and duodenal homeobox 1(PDX-1) induction in treating type 1 diabetic SD rats.MethodsThe third (P3) and fifth (P5) generation of BMSCs were transfected with PDX-1 and induced to islet-like cells. Dithizone staining was used to detect insulin secretion. Streptozotocin was intraperitoneally injected into SD rats to induce type 1 diabetic model. The 45 established diabetic SD rat models were divided into three groups with random number table(15 rats in each group): transplantation group A, transplantation group B and model control group, the three groups were injected with islet-like cells derived from P3BMSCs+PDX-1, P5 BMSCs+PDX-1 and saline through tail-vain, respectively. Another 15 healthy SD rats with tail-vein injection of equal volume of saline were set as normal controls. Blood glucose, fasting insulin and C-peptide levels were measured at 0, 7, 14, 21 and 28 days after transplantation. Intraperitoneal glucose tolerance test (IPGTT) was conducted at the 21st day after transplantation. Rats were euthanized at day 28 and pancreatic tissues were collected and tested by immunohistochemistry. One-way analysis of variance was applied to do statistic analysis.ResultsFrom the 14th day of transplantation on, blood glucose levels significantly decreased in rats from group A and group B when compared with those in model control group. Fasting insulin and C - peptide levels in transplantation groups increased, although the difference was not statistically significant. The blood glucose levels in group B on the 21th and 28th day were all significantly lower than those in group A ((21.90±0.26) vs (23.60±1.49) mmol/L, (21.80±1.32) vs (24.20±2.06) mmol/L, t=9.65, 12.73, both P<0.05). Consistently, plasma insulin and C-peptide levels were higher in group B than those in group A at the corresponding time points(t=8.73, 12.51, 18.36, 25.12, all P<0.05). IPGTT showed that the area under curve in group B was significantly lower than that in group A ((1 809.0±2.6) vs (2 301.0±6.8) mmol·L-1·min, t=5.241, P<0.05). Pancreatic immunohistochemistry showed that insulin positive cells were detected in both transplantation group A and B; and the percentage of insulin positive cells were higher in group B than that in Group A(48.0% ± 1.3% vs 32.8% ± 3.2%, t=8.38, P<0.05).ConclusionIslet-like cells originated from the 5th generation of BMSCs with PDX-1 induction have better effects than the 3rd generation in treating type 1 diabetic rats.Key words: Diabetes mellitus, type 1; Pancreatic and duodenal homeobox 1; Bone marrow mesenchymal stem cells; Insulin-producing cells" @default.
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- W3030247948 date "2016-07-27" @default.
- W3030247948 modified "2023-09-23" @default.
- W3030247948 title "Effects of islet-like cells differentiated from different generations of rat bone mesenchymal stem cells with pancreatic and duodenal homeobox 1 induction in treating diabetic rats" @default.
- W3030247948 doi "https://doi.org/10.3760/cma.j.issn.1674-5809.2016.07.010" @default.
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