Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030254059> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3030254059 endingPage "809" @default.
- W3030254059 startingPage "805" @default.
- W3030254059 abstract "Objective To estimate the effect of glycoprotein (transmembrane) nonmetastatic melanoma protein B (GPNMB) on the proliferation and migration of as well as melanogenesis in melanoma cells.Methods The expression of GPNMB was detected by immunofluorescence assay in two melanoma cell lines M14 and G-361,as well as in primary human melanocytes.Then,the three kinds of cells each were classified into three groups:experimental group treated with small interfering RNA targeting GPNMB (GPNMB-siRNA),negative control group treated with the negative control siRNA,blank control group remaining untreated.Methyl thiazolyl tetrazolium (MTT) assay,transwell invasion assay and spectrophotometry were performed to evaluate cell proliferation activity,invasion potential and melanin levels,respectively.Statistical analysis was done using Student's t test.Results GPNMB was expressed in both melanoma cells and melanocytes.The transfection with GPNMB-siRNA down-regulated the mRNA and protein expressions of GPNMB in,and markedly suppressed the proliferation and migration of,melanoma cells.In detail,the proliferative activity (expressed as the absorbence value at 570 nm) of M14 and G361 cells was reduced by 35％ and 40％ respectively,the migration activity of M14 and G361 cells by 49％ and 51％ respectively,and the melanin levels in melanocytes,M14 cells and G361 cells by 73％,82％ and 69％ respectively,in the experiment group compared with those in the blank control group.Conclusions The siRNA-mediated silencing of GPNMB could effectively inhibit the proliferation of,invasion of and melanogenesis in melanoma cells,which suggests that GPNMB plays critical roles in the initiation and progression of melanoma.Key words: GPNMB; RNA, small interfering; Melanins; Cell proliferation; Cell movement" @default.
- W3030254059 created "2020-06-05" @default.
- W3030254059 creator A5055151897 @default.
- W3030254059 creator A5055989962 @default.
- W3030254059 creator A5058034372 @default.
- W3030254059 creator A5071037763 @default.
- W3030254059 creator A5087272361 @default.
- W3030254059 date "2013-11-15" @default.
- W3030254059 modified "2023-09-24" @default.
- W3030254059 title "Effect of glycoprotein (transmembrane) nonmetastatic melanoma protein B on the proliferation and migration of as well as melanogenesis in melanoma cells" @default.
- W3030254059 doi "https://doi.org/10.3760/cma.j.issn.0412-4030.2013.11.010" @default.
- W3030254059 hasPublicationYear "2013" @default.
- W3030254059 type Work @default.
- W3030254059 sameAs 3030254059 @default.
- W3030254059 citedByCount "0" @default.
- W3030254059 crossrefType "journal-article" @default.
- W3030254059 hasAuthorship W3030254059A5055151897 @default.
- W3030254059 hasAuthorship W3030254059A5055989962 @default.
- W3030254059 hasAuthorship W3030254059A5058034372 @default.
- W3030254059 hasAuthorship W3030254059A5071037763 @default.
- W3030254059 hasAuthorship W3030254059A5087272361 @default.
- W3030254059 hasConcept C104317684 @default.
- W3030254059 hasConcept C107538193 @default.
- W3030254059 hasConcept C119056186 @default.
- W3030254059 hasConcept C11957725 @default.
- W3030254059 hasConcept C137738243 @default.
- W3030254059 hasConcept C1491633281 @default.
- W3030254059 hasConcept C153911025 @default.
- W3030254059 hasConcept C185592680 @default.
- W3030254059 hasConcept C22615655 @default.
- W3030254059 hasConcept C2776415932 @default.
- W3030254059 hasConcept C2777658100 @default.
- W3030254059 hasConcept C2779769559 @default.
- W3030254059 hasConcept C502942594 @default.
- W3030254059 hasConcept C54009773 @default.
- W3030254059 hasConcept C54355233 @default.
- W3030254059 hasConcept C55493867 @default.
- W3030254059 hasConcept C62112901 @default.
- W3030254059 hasConcept C81885089 @default.
- W3030254059 hasConcept C86803240 @default.
- W3030254059 hasConceptScore W3030254059C104317684 @default.
- W3030254059 hasConceptScore W3030254059C107538193 @default.
- W3030254059 hasConceptScore W3030254059C119056186 @default.
- W3030254059 hasConceptScore W3030254059C11957725 @default.
- W3030254059 hasConceptScore W3030254059C137738243 @default.
- W3030254059 hasConceptScore W3030254059C1491633281 @default.
- W3030254059 hasConceptScore W3030254059C153911025 @default.
- W3030254059 hasConceptScore W3030254059C185592680 @default.
- W3030254059 hasConceptScore W3030254059C22615655 @default.
- W3030254059 hasConceptScore W3030254059C2776415932 @default.
- W3030254059 hasConceptScore W3030254059C2777658100 @default.
- W3030254059 hasConceptScore W3030254059C2779769559 @default.
- W3030254059 hasConceptScore W3030254059C502942594 @default.
- W3030254059 hasConceptScore W3030254059C54009773 @default.
- W3030254059 hasConceptScore W3030254059C54355233 @default.
- W3030254059 hasConceptScore W3030254059C55493867 @default.
- W3030254059 hasConceptScore W3030254059C62112901 @default.
- W3030254059 hasConceptScore W3030254059C81885089 @default.
- W3030254059 hasConceptScore W3030254059C86803240 @default.
- W3030254059 hasIssue "11" @default.
- W3030254059 hasLocation W30302540591 @default.
- W3030254059 hasOpenAccess W3030254059 @default.
- W3030254059 hasPrimaryLocation W30302540591 @default.
- W3030254059 hasRelatedWork W2069747971 @default.
- W3030254059 hasRelatedWork W2188463817 @default.
- W3030254059 hasRelatedWork W2264667793 @default.
- W3030254059 hasRelatedWork W2350109665 @default.
- W3030254059 hasRelatedWork W2353298330 @default.
- W3030254059 hasRelatedWork W2354640678 @default.
- W3030254059 hasRelatedWork W2362972375 @default.
- W3030254059 hasRelatedWork W2377941619 @default.
- W3030254059 hasRelatedWork W2378270447 @default.
- W3030254059 hasRelatedWork W2385108197 @default.
- W3030254059 hasRelatedWork W2385439823 @default.
- W3030254059 hasRelatedWork W2793935128 @default.
- W3030254059 hasRelatedWork W3028683394 @default.
- W3030254059 hasRelatedWork W3028953840 @default.
- W3030254059 hasRelatedWork W3029781303 @default.
- W3030254059 hasRelatedWork W3030432786 @default.
- W3030254059 hasRelatedWork W3030979391 @default.
- W3030254059 hasRelatedWork W3031596007 @default.
- W3030254059 hasRelatedWork W3031886609 @default.
- W3030254059 hasRelatedWork W2622004512 @default.
- W3030254059 hasVolume "46" @default.
- W3030254059 isParatext "false" @default.
- W3030254059 isRetracted "false" @default.
- W3030254059 magId "3030254059" @default.
- W3030254059 workType "article" @default.