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- W3030286802 abstract "Objective To study expression of Izumo mRNA in testis and epididymis of Inner Mongolian white cashmere goats.Methods Part of the Izumo gene fragment was recloned by reverse transcription-polymerase chain reaction (RT-PCR).Then another pair of primers were designed.Digoxigeninlabeled DNA probes were prepared and hybridized with total RNA extracted from tissues of testis and head,body and tail of epididymis by Northern blotting.Results The size of the amplified fragment by RT-PCR was 788 bp as expected.The concentration of the labeled DNA probe was 100 pg/μl and expression of Izumo mRNA was identified in tissues of testis and head,body and tail of epididymis.Conclusion The testis and epididymis of Inner Mongolian white cashmere goats can express Izumo mRNA,which lays foundation for further study of roles of Izumo in human sperm-egg fusion.Key words: Izumo gene; Inner Mongolian white cashmere goat; Testis; Epididymis; Blotting, Northern" @default.
- W3030286802 created "2020-06-05" @default.
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- W3030286802 date "2014-04-10" @default.
- W3030286802 modified "2023-09-23" @default.
- W3030286802 title "Expression of Izumo mRNA in testis and epididymis of Inner Mongolian white cashmere goats" @default.
- W3030286802 doi "https://doi.org/10.3760/cma.j.issn.1673-4211.2014.02.003" @default.
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