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• W30303934 abstract "Publisher Summary The RNA segment 8 of influenza A virus codes for two overlapping polypeptides that are synthesized during viral infection and are not present in mature virions. A number of influenza Viral proteins, including hemagglutinin (HA), neuraminidase, nucleoprotein (NP), and matrix (M) protein, have been successfully expressed in mammalian cells by viral vectors containing cloned influenza viral cDNA copies. This chapter discusses the analysis of a host range mutant of influenza virus with a deletion in the NS gene segment and the successful expression of NSl protein from cloned cDNA in CV-1 cells using an SV40 viral vector. In the analysis, each plasmid was digested to remove the pBR322 sequences and relegated to produce circular SV40-NS recombinant molecules. Each DNA molecule was then co-transfected into CV-1 cells along with a helper SV40 recombinant. This recombinant pSV-r-INS-7 is an SV40 vector that has the insulin gene replacing portions of the early region of SV40. After a two week incubation period, cells were freeze thawed three times and the supernatant was used as the viral stock." @default.
• W30303934 created "2016-06-24" @default.
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• W30303934 date "1984-01-01" @default.
• W30303934 modified "2023-09-27" @default.
• W30303934 title "THE NONSTRUCTURAL GENE SEGMENT OF INFLUENZA A VIRUS: EXPRESSION OF NS1 PROTEIN IN MAMMALIAN CELLS; ANALYSIS OF A DELETION MUTANT" @default.
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• W30303934 doi "https://doi.org/10.1016/b978-0-12-183501-9.50026-1" @default.
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