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- W3030436615 abstract "目的手术应激可引起细胞介导的免疫受到抑制,包括淋巴细胞数量减少,导致感染并发症增加,确切的原因尚不清楚,本研究探讨婴幼儿大手术损伤和淋巴细胞凋亡的关系.方法12例择期大手术婴幼儿和6例严重感染的婴幼儿,择期手术患儿包括先天性胆总管囊肿7例和先天性巨结肠5例,其中男8例,女4例,年龄6个月～3岁;6例正常婴幼儿作为对照,抽取外周血,用Boyum描述的方法借助梯度离心分离淋巴细胞,用Annexin V/PI双染色法来分析淋巴细胞早期凋亡情况,流式细胞仪测定淋巴细胞Fas的表达.结果术后患儿外周血淋巴细胞明显下降,术后1 d为最低,术后3d仍处于低值.大手术组患儿淋巴细胞凋亡率为(14.1±1.1)%,严重感染组为(12.3±2.0)%,与正常对照组(5.1±0.3)%相比有统计学意义(P＜0.05);Fas阳性淋巴细胞百分数术毕为(50.3±6.3)%,术后1 d为(49.3±4.3)%,与术前(36.3±1.1%)相比有统计学意义(P＜0.05).结论经历大手术的患儿循环淋巴细胞呈现凋亡的早期征象,可能是全身炎症反应中免疫细胞低反应的原因." @default.
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- W3030436615 date "2003-07-15" @default.
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- W3030436615 title "Relation between surgical injury and apoptosis of peripheral lymphocytes in infants" @default.
- W3030436615 doi "https://doi.org/10.3760/cma.j.issn.0253-3006.2003.04.001" @default.
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