Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030483921> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3030483921 endingPage "286" @default.
- W3030483921 startingPage "279" @default.
- W3030483921 abstract "ObjectiveTo construct the expression profiles of microRNA (miRNA) and mRNA in ovarian granulosa cells in patients with polycystic ovary syndrome (PCOS) in search of the molecular markers of PCOS.MethodsA standard length scheme was used to collect ovarian granulosa cells of PCOS patients (PCOS group) and normal ovulating women (control group) who received in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) in the Reproductive Medicine Center of the Affiliated Hospital of Inner Mongolia Medical University from 2017 to 2018, integrate high-throughput mRNA and miRNA expression profiles, and the biological processes involved in differential expression genes were analyzed through Gene Ontogy's functional enrichment and KEGG Pathway. At the same time, the core regulatory network during the pathogenesis of PCOS was constructed by using the relationship between miRNA target regulatory genes, and PCOS-related mRNAs and miRNAs were predicted.ResultsCompared with control group, 66 miRNAs with differential expression were screened in POCS group, among which 42 miRNAs were up-regulated and 24 miRNAs were down-regulated. Totally 416 differentially expressed genes were screened by transcription profiling, among which 236 genes were up-regulated and 180 genes were down-regulated (P<0.05, |log2FC|≥2). Highly significantly differentially expressed miRNAs (miRNA-26b, miRNA-423-3p, miRNA-219a, miRNA-326, miRNA-3928-3p and miRNA-194-5p) were associated with 107 differentially targeted genes by joint analysis, with significant differences (P<0.01). These target genes involve many functions such as ion transport, cell adhesion and inflammatory reactions, and multiple signal pathways such as mitogen-activated protein kinase (MAPK), Wnt and phosphateidy-linositol-3-kinase/serine-threonine kinase (PI3K-Akt).ConclusionThe expression of miRNA and its regulatory target genes in ovarian granulosa cells in patients with PCOS are closely related to their pathophysiological processes.Key words: Polycystic ovary syndrome; High-throughput sequencing; Ovarian granulosa cells; mRNA; MicroRNAs" @default.
- W3030483921 created "2020-06-05" @default.
- W3030483921 creator A5022903895 @default.
- W3030483921 creator A5047041829 @default.
- W3030483921 creator A5075529271 @default.
- W3030483921 creator A5076682461 @default.
- W3030483921 creator A5083142408 @default.
- W3030483921 date "2020-04-14" @default.
- W3030483921 modified "2023-09-22" @default.
- W3030483921 title "Construction of microRNA and mRNA expression profiles in granulosa cells of patients with polycystic ovary syndrome" @default.
- W3030483921 doi "https://doi.org/10.3760/cma.j.cn101441-20190529-00216" @default.
- W3030483921 hasPublicationYear "2020" @default.
- W3030483921 type Work @default.
- W3030483921 sameAs 3030483921 @default.
- W3030483921 citedByCount "0" @default.
- W3030483921 crossrefType "journal-article" @default.
- W3030483921 hasAuthorship W3030483921A5022903895 @default.
- W3030483921 hasAuthorship W3030483921A5047041829 @default.
- W3030483921 hasAuthorship W3030483921A5075529271 @default.
- W3030483921 hasAuthorship W3030483921A5076682461 @default.
- W3030483921 hasAuthorship W3030483921A5083142408 @default.
- W3030483921 hasConcept C104317684 @default.
- W3030483921 hasConcept C119056186 @default.
- W3030483921 hasConcept C134018914 @default.
- W3030483921 hasConcept C145059251 @default.
- W3030483921 hasConcept C150194340 @default.
- W3030483921 hasConcept C152724338 @default.
- W3030483921 hasConcept C162317418 @default.
- W3030483921 hasConcept C16685009 @default.
- W3030483921 hasConcept C18431079 @default.
- W3030483921 hasConcept C2777203538 @default.
- W3030483921 hasConcept C2777391703 @default.
- W3030483921 hasConcept C2778324911 @default.
- W3030483921 hasConcept C2779306644 @default.
- W3030483921 hasConcept C3018442814 @default.
- W3030483921 hasConcept C54355233 @default.
- W3030483921 hasConcept C71924100 @default.
- W3030483921 hasConcept C86803240 @default.
- W3030483921 hasConceptScore W3030483921C104317684 @default.
- W3030483921 hasConceptScore W3030483921C119056186 @default.
- W3030483921 hasConceptScore W3030483921C134018914 @default.
- W3030483921 hasConceptScore W3030483921C145059251 @default.
- W3030483921 hasConceptScore W3030483921C150194340 @default.
- W3030483921 hasConceptScore W3030483921C152724338 @default.
- W3030483921 hasConceptScore W3030483921C162317418 @default.
- W3030483921 hasConceptScore W3030483921C16685009 @default.
- W3030483921 hasConceptScore W3030483921C18431079 @default.
- W3030483921 hasConceptScore W3030483921C2777203538 @default.
- W3030483921 hasConceptScore W3030483921C2777391703 @default.
- W3030483921 hasConceptScore W3030483921C2778324911 @default.
- W3030483921 hasConceptScore W3030483921C2779306644 @default.
- W3030483921 hasConceptScore W3030483921C3018442814 @default.
- W3030483921 hasConceptScore W3030483921C54355233 @default.
- W3030483921 hasConceptScore W3030483921C71924100 @default.
- W3030483921 hasConceptScore W3030483921C86803240 @default.
- W3030483921 hasIssue "04" @default.
- W3030483921 hasLocation W30304839211 @default.
- W3030483921 hasOpenAccess W3030483921 @default.
- W3030483921 hasPrimaryLocation W30304839211 @default.
- W3030483921 hasRelatedWork W2069736363 @default.
- W3030483921 hasRelatedWork W2070806164 @default.
- W3030483921 hasRelatedWork W2410152695 @default.
- W3030483921 hasRelatedWork W2556030154 @default.
- W3030483921 hasRelatedWork W2766792020 @default.
- W3030483921 hasRelatedWork W2771091682 @default.
- W3030483921 hasRelatedWork W2782167959 @default.
- W3030483921 hasRelatedWork W2790513846 @default.
- W3030483921 hasRelatedWork W2793824550 @default.
- W3030483921 hasRelatedWork W2805961026 @default.
- W3030483921 hasRelatedWork W2942837101 @default.
- W3030483921 hasRelatedWork W2951099267 @default.
- W3030483921 hasRelatedWork W2988980787 @default.
- W3030483921 hasRelatedWork W2991767540 @default.
- W3030483921 hasRelatedWork W3006167540 @default.
- W3030483921 hasRelatedWork W3127106656 @default.
- W3030483921 hasRelatedWork W3131770527 @default.
- W3030483921 hasRelatedWork W3160971709 @default.
- W3030483921 hasRelatedWork W3165471124 @default.
- W3030483921 hasRelatedWork W3182818044 @default.
- W3030483921 hasVolume "40" @default.
- W3030483921 isParatext "false" @default.
- W3030483921 isRetracted "false" @default.
- W3030483921 magId "3030483921" @default.
- W3030483921 workType "article" @default.