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- W3030485588 abstract "Objecfive To investigate the effects of acute or chronic metabolic acidosis on intracellular pH of rat erythrocytes.Methods Acute metabolic acidosis in Wistar rats was induced by infusion of 4mmol/kg HCl for 4 hours;Chronic metabolic acidosis rats was induced by addition of 0.28 mol/L NH4CI to drinking water for 7 days.The control groups were given 0.9%NaCl in the same time.At 0,2and 4h after HCl infusion in acute group,and at 0,1,3,5 and 7 days after NH4Cl administration in chronic group,0.5ml blood samples were taken.All blood samples were placed in test tubes with heparin anticoagulant solution.Blood gas was analyzed.Carbonic anhydrase activity in rat erythrocytes was assayed by following the hydration of CO2 according to the method described by Wilbur and Anderson.And after stained by BCECF-AM fluorescent probe,the changes of intracellular pH were observed,and the ability of NHE1 were measured by detected recovery rate of intraeellular pH by laser scanning confocal microscopy.Results Acute metabolic acidosis inhibits NHE1 activity(P＜0.05)and no changes in pHi and carbonic anhydrase activity of rat erythrocyte were seen.On the contrast.it was showed increasing activity of NHE 1 and carbonic anhydrase with decreasing phi in rats erythrocytesin chronic metabolic acidosis over five days(P＜0.05).Conclusion These results suggest that an acute acid load does not alter pHi while chronic metabolic acidosis does reduce pHi of rat crythrocyte.Key words: Metabolic acidosis; Carbonic anhydrase; Na+-H+exchanger I; Erythrocyte; Intracellular pH" @default.
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- W3030485588 date "2009-10-15" @default.
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- W3030485588 title "Effects of acute or chronic metabolic acidosis on intracellular pH of rat erythrocytes" @default.
- W3030485588 doi "https://doi.org/10.3760/cma.j.issn.1673-4378.2009.05.012" @default.
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