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- W3030489711 abstract "ObjectiveTo analyze the expression of YTH domain containing family 2 (YTHDF2) in the hepatocellular carcinom (HCC) and the relationship between the expression of YTHDF2 and prognosis. To detect the influence of YTHDF2 in cell proliferation, migration and invasion.MethodsWe detected the expression of YTHDF2 among the paired HCC tissue and adjacent tumor tissue with bioinformatics, real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and immunohistochemistry. To analyze the relationship between YTHDF2 expression and clinical characteristics. We established the HCC cells with YTHDF2 knockdown and analyzed the cell proliferation with cell counting kit-8 (CCK-8) assay and detect the cell migration with Transwell and wound scratch assay. To analyze the expression of E-cadherin and N-cadherin that associated with epithelial-mesenchymal transition (EMT) with western blotting.ResultsBioinformatics analysis showed the high expression of YTHDF2 in HCC tumor and its relationship with poor prognosis. We found that the HCC tumor tissue has a higher expression of YTHDF2 in compared with non-tumor adjacent tissue (17.76±4.78 vs. 12.10±3.43, P<0.01). The expression of YTHDF2 is positively related with BCLC stage, TNM stage and portal vein tumor thrombosis (PVTT) (P<0.01). CCK-8 assay demonstrated that the absorbance at 450 nm of the cell with YTHDF2 knockdown is lower than the negative control, while HepG2 is (1.129±0.178 vs. 1.571±0.226, P<0.01) and Huh7 (1.227±0.251 vs. 1.815±0.302, P<0.05). Transwell assay showed that the number of cell that diffuses through the membrane of cell with YTHDF2 knockdown is lower than the negative control. The wound scratch assay showed that the percent of wound area in cell with YTHDF2 knockdown is lower. We detected the HCC cells with YTHDF2 konckdown and found it had a higher relative expression of E-cadherin (1.780±0.319 vs. 0.916±0.173, P<0.05) and a lower expression of N-cadherin (0.821±0.217 vs. 4.697±0.398, P<0.01).ConclusionThe high expression of YTHDF2 in HCC tissue showed its relationship with HCC development and YTHDF2 may play an important role in HCC cell proliferation and migration.Key words: YTH domain containing family 2; Hepatocellular carcinom; Epithelial-mesenchymal transition" @default.
- W3030489711 created "2020-06-05" @default.
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- W3030489711 date "2019-02-08" @default.
- W3030489711 modified "2023-09-26" @default.
- W3030489711 title "Expression andclinical significance of YTH domain containing family 2 in hepatocellular carcinoma" @default.
- W3030489711 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2019.02.040" @default.
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