Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030489740> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3030489740 endingPage "1192" @default.
- W3030489740 startingPage "1189" @default.
- W3030489740 abstract "Objective To study the relationship between the expression of p53 and its downstream effecter p21^wafl protein and multidrug resistance （MDR） of Tca8113/BLM cell line. Methods The cDNA of wildtype p53 gene was introduced into the drug-resistance cell line Tca8113/BLM by Lipofectamine-mediated transfection. The expression of p53 mRNA in Tca8113/BLM and its parental counterpart Tca8113 cell lines were analyzed using molecular beacons. The expression of p53, p21^wafl , P-gp, and MRP proteins in Tca8113/BLM cell line transfected by wt-p53 cDNA ,the untransfected control Tca8113/BLM cell line, its parental counterpart Tca8113 were analyzed by Western blotting. MTT assay was used to determine the drug-sensitivity of different cell lines. The cell cycle distribution and apotosis of different cell lines were also observed by flow cytometery （ FCM ） and laser confocus microscope （ LCM ）, respectively. Results The expression of p53 protein was significantly lower, and no p21^wafl protein was detected in Tca8113/BLM cells. The protein expression of P-gp and MRP were significantly higher in Tca8113/BLM cells than in Tca8113 cells. By Western Blotting, the expression of p53 and p21^wafl proteins was significantly increased, while the expression of P-gp and MRP proteins was significantly decreased in Tca8113/BLM/p53 cells. Comparing to Tca8113/BLM cells, the sensitivity of BLM treatment in Tca8113/BLM/p53 cells was 10. 1 fold increased. Tca8113/BLM/p53 cells decreased at G1 and S phase, and increased at G2 phase. LCM results showed that the apoptosis of Tca8113/BLM/p53 cells was more obvious than Tca8113/BLM cells when treated with the drug BLM. Conclusion The multi-drug resistance in Tca8113/BLM cell line is involved in down-regulation of p53 and p21^wafl protein levels, and up-regulation of P-gp and MRP protein levels.Key words: Tongue neoplasms ; Drug resistance, neoplasm ; Protein p53 ; Oncogene protein p21 （ras）" @default.
- W3030489740 created "2020-06-05" @default.
- W3030489740 creator A5009962981 @default.
- W3030489740 creator A5024326996 @default.
- W3030489740 creator A5047120567 @default.
- W3030489740 creator A5048751341 @default.
- W3030489740 creator A5049827502 @default.
- W3030489740 creator A5053594758 @default.
- W3030489740 date "2006-09-10" @default.
- W3030489740 modified "2023-09-25" @default.
- W3030489740 title "Relationship between the expression of p53 and its downstream effecter p21^wafl proteins and multi-drug resistance of human tongue cancer cell line" @default.
- W3030489740 doi "https://doi.org/10.3760/cma.j.issn.1008-1372.2006.09.015" @default.
- W3030489740 hasPublicationYear "2006" @default.
- W3030489740 type Work @default.
- W3030489740 sameAs 3030489740 @default.
- W3030489740 citedByCount "0" @default.
- W3030489740 crossrefType "journal-article" @default.
- W3030489740 hasAuthorship W3030489740A5009962981 @default.
- W3030489740 hasAuthorship W3030489740A5024326996 @default.
- W3030489740 hasAuthorship W3030489740A5047120567 @default.
- W3030489740 hasAuthorship W3030489740A5048751341 @default.
- W3030489740 hasAuthorship W3030489740A5049827502 @default.
- W3030489740 hasAuthorship W3030489740A5053594758 @default.
- W3030489740 hasConcept C104317684 @default.
- W3030489740 hasConcept C1491633281 @default.
- W3030489740 hasConcept C153911025 @default.
- W3030489740 hasConcept C185592680 @default.
- W3030489740 hasConcept C187882448 @default.
- W3030489740 hasConcept C190283241 @default.
- W3030489740 hasConcept C29537977 @default.
- W3030489740 hasConcept C40767141 @default.
- W3030489740 hasConcept C49923114 @default.
- W3030489740 hasConcept C54009773 @default.
- W3030489740 hasConcept C54355233 @default.
- W3030489740 hasConcept C55493867 @default.
- W3030489740 hasConcept C81885089 @default.
- W3030489740 hasConcept C85265743 @default.
- W3030489740 hasConcept C86803240 @default.
- W3030489740 hasConcept C92087593 @default.
- W3030489740 hasConceptScore W3030489740C104317684 @default.
- W3030489740 hasConceptScore W3030489740C1491633281 @default.
- W3030489740 hasConceptScore W3030489740C153911025 @default.
- W3030489740 hasConceptScore W3030489740C185592680 @default.
- W3030489740 hasConceptScore W3030489740C187882448 @default.
- W3030489740 hasConceptScore W3030489740C190283241 @default.
- W3030489740 hasConceptScore W3030489740C29537977 @default.
- W3030489740 hasConceptScore W3030489740C40767141 @default.
- W3030489740 hasConceptScore W3030489740C49923114 @default.
- W3030489740 hasConceptScore W3030489740C54009773 @default.
- W3030489740 hasConceptScore W3030489740C54355233 @default.
- W3030489740 hasConceptScore W3030489740C55493867 @default.
- W3030489740 hasConceptScore W3030489740C81885089 @default.
- W3030489740 hasConceptScore W3030489740C85265743 @default.
- W3030489740 hasConceptScore W3030489740C86803240 @default.
- W3030489740 hasConceptScore W3030489740C92087593 @default.
- W3030489740 hasIssue "09" @default.
- W3030489740 hasLocation W30304897401 @default.
- W3030489740 hasOpenAccess W3030489740 @default.
- W3030489740 hasPrimaryLocation W30304897401 @default.
- W3030489740 hasRelatedWork W1151369203 @default.
- W3030489740 hasRelatedWork W2021921377 @default.
- W3030489740 hasRelatedWork W2086515636 @default.
- W3030489740 hasRelatedWork W2353345506 @default.
- W3030489740 hasRelatedWork W2355060871 @default.
- W3030489740 hasRelatedWork W2356206256 @default.
- W3030489740 hasRelatedWork W2358897836 @default.
- W3030489740 hasRelatedWork W2368324296 @default.
- W3030489740 hasRelatedWork W2368480798 @default.
- W3030489740 hasRelatedWork W2370059661 @default.
- W3030489740 hasRelatedWork W2376041115 @default.
- W3030489740 hasRelatedWork W2381461172 @default.
- W3030489740 hasRelatedWork W2395351287 @default.
- W3030489740 hasRelatedWork W2418394189 @default.
- W3030489740 hasRelatedWork W2427309985 @default.
- W3030489740 hasRelatedWork W2438095353 @default.
- W3030489740 hasRelatedWork W3032420735 @default.
- W3030489740 hasRelatedWork W3032668221 @default.
- W3030489740 hasRelatedWork W3108162360 @default.
- W3030489740 hasRelatedWork W3211787509 @default.
- W3030489740 hasVolume "8" @default.
- W3030489740 isParatext "false" @default.
- W3030489740 isRetracted "false" @default.
- W3030489740 magId "3030489740" @default.
- W3030489740 workType "article" @default.