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• W3030514370 abstract "ObjectiveTo investigate the regulatory role and its mechanism of transforming growth factor-β (TGF-β)/Smad pathway on the balance of helper T cell 17/T-regulatory cells (Th17/Treg) in a mouse model of acute lung injury (ALI). MethodsSixty C57BL/6 mice were divided into three groups according to randomize number table method: control group (n = 20), ALI model group (n = 20), and interleukin-17 (IL-17) antibody (Ab) pretreatment group (n = 20). The mice in IL-17 Ab pretreatment group were delivered 200 μg IL-17 Ab intraperitoneally, while the ALI model group were given 200 μg Iso-IgG2a. Both groups were challenged by 4 mg/kg lipopolysaccharides (LPS) [dissolved in 30 μL phosphate buffer saline (PBS)] intratracheally to induce ALI. The mice in control group were given 30 μL PBS. Twenty-four hours later, all mice were sacrificed and lung tissues and bronchoalveolar lavage fluid (BALF) were harvested. The pathological images were observed under light microscope, the lung wet weight/dry weight (W/D) ratio and the protein content in BALF were measured to assess the severity of lung injury. The levels of IL-6, IL-10, IL-17A and TGF-β1 in lung homogenates were assessed by enzyme-linked immunosorbent assay (ELISA). The ratio of Treg and Th17 was examined by flow cytometer. The mRNA expression levels of retinoid-related orphan nuclear receptor γt (ROR-γt), TGF-β1, Foxp3, Smad2, Smad3 and Smad7 were quantitatively measured by real-time polymerase chain reaction (PCR). The phosphates Smad2/3 and Smad7 (p-Smad2/3, p-Smad7) of lung tissues were detected by Western Blot. ResultsCompared with the control group, a large number of inflammatory cells infiltration could be observed and accompanied by alveolar septal thickening, alveolar congestion and edema/collapse and dot bleeding; the ratio of W/D, the protein levels in BALF, the concentrations of IL-6, IL-17A and TGF-β1 in lung tissue homogenates, the ratio of Th17/Treg and the mRNA expressions of ROR-γt, TGF-β1, Foxp3, Smad2, and Smad3 were significantly elevated, the mRNA expression of Smad7 was significantly decreased in the ALI model group (all P 0.05) was similar. There were no significant differences in protein levels of IL-10 of lung tissue among all the groups. ConclusionThe imbalance of Th17/Treg may play an important role in the immunological mechanism of ALI, which may be partially modulated by TGF-β/Smad pathway.Key words: Acute lung injury; Helper T cell 17; T-regulatory cell; Transforming growth factor-β1; Smad" @default.
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• W3030514370 date "2016-11-10" @default.
• W3030514370 modified "2023-09-23" @default.
• W3030514370 title "Regulatory role of transforming growth factor-β/Smad pathway on immune imbalance in a mouse model of acute lung injury" @default.
• W3030514370 doi "https://doi.org/10.3760/cma.j.issn.2095-4352.2016.11.004" @default.
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