Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030522011> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3030522011 endingPage "200" @default.
- W3030522011 startingPage "196" @default.
- W3030522011 abstract "目的 探讨细胞信号转导信使磷脂酰肌醇3激酶(PI3K)在卵巢上皮性癌(卵巢癌)组织中的表达及其意义;并探讨PI3K抑制剂LY294002联合顺铂对卵巢癌细胞株的生长抑制作用.方法 应用蛋白印迹法和RT-PCR技术检测正常卵巢组织(正常组,20份)、卵巢良性上皮性肿瘤组织(良性组,6份)、卵巢交界性上皮性肿瘤组织(交界性组,6份)和卵巢癌组织(卵巢癌组,39份)中PI3K p85亚单位蛋白和mRNA的表达.将SKOV3细胞分为对照组(只加培养液)、LY294002组(加1、10、30、50、100 μmol/L LY294002)、顺铂组(加0.33、1.25、2.5、5、10 μmol/L顺铂)和联合组(加50 μmol/L LY294002+10 μmol/L顺铂),四甲基偶氮唑蓝还原法测定不同浓度的LY294002及顺铂对SKOV3细胞的生长抑制作用.结果 (1)PI3K p85亚单位蛋白的表达阳性率:正常组和良性组均为0,交界性组为2/6,卵巢癌组为85%(33/39),正常组、良性组、交界性组分别与卵巢癌组比较,差异均有统计学意义(P＜0.01).(2)PI3K p85亚单位mRNA的表达水平:正常组为0.178±0.102,良性组为0.643±0.112,交界性组为0.847±0.058,卵巢癌组为1.689±0.423,正常组、良性组、交界性组分别与卵巢癌组比较,差异均有统计学意义(P＜0.01).卵巢癌组织中,PI3K p85亚单位蛋白表达阳性率及mRNA的表达水平,不同年龄、病理类型间比较,差异均无统计学意义(P＞0.05);而不同病理分化程度、手术病理分期间比较,差异均有统计学意义(P＜0.05).(3)LY294002及顺铂呈剂量依赖性地抑制SKOV3细胞的生长.LY294002组(50 μmol/L)、顺铂组(10 μmol/L)、联合组SKOV3细胞的抑制率分别为(46.0±2.0)%、(44.4±3.2)%、(57.1±4.1)%,联合组SKOV3细胞的抑制率高于LY294002组及顺铂组(P＜0.01).结论 PI3K p85亚单位在卵巢癌组织中呈高表达,与病理分化程度、手术病理分期有关.LY294002与顺铂联合用药对卵巢癌细胞生长的抑制具有协同效应。" @default.
- W3030522011 created "2020-06-05" @default.
- W3030522011 creator A5012901863 @default.
- W3030522011 creator A5067582895 @default.
- W3030522011 date "2007-03-25" @default.
- W3030522011 modified "2023-09-25" @default.
- W3030522011 title "Expression of phosphatidylinositol-3 kinase in epithelial ovarian carcinoma" @default.
- W3030522011 doi "https://doi.org/10.3760/j.issn:0529-567x.2007.03.014" @default.
- W3030522011 hasPublicationYear "2007" @default.
- W3030522011 type Work @default.
- W3030522011 sameAs 3030522011 @default.
- W3030522011 citedByCount "0" @default.
- W3030522011 crossrefType "journal-article" @default.
- W3030522011 hasAuthorship W3030522011A5012901863 @default.
- W3030522011 hasAuthorship W3030522011A5067582895 @default.
- W3030522011 hasConcept C121608353 @default.
- W3030522011 hasConcept C126322002 @default.
- W3030522011 hasConcept C184235292 @default.
- W3030522011 hasConcept C199360897 @default.
- W3030522011 hasConcept C2780427987 @default.
- W3030522011 hasConcept C2780610907 @default.
- W3030522011 hasConcept C3019054536 @default.
- W3030522011 hasConcept C41008148 @default.
- W3030522011 hasConcept C502942594 @default.
- W3030522011 hasConcept C71924100 @default.
- W3030522011 hasConcept C86803240 @default.
- W3030522011 hasConcept C90559484 @default.
- W3030522011 hasConcept C95444343 @default.
- W3030522011 hasConceptScore W3030522011C121608353 @default.
- W3030522011 hasConceptScore W3030522011C126322002 @default.
- W3030522011 hasConceptScore W3030522011C184235292 @default.
- W3030522011 hasConceptScore W3030522011C199360897 @default.
- W3030522011 hasConceptScore W3030522011C2780427987 @default.
- W3030522011 hasConceptScore W3030522011C2780610907 @default.
- W3030522011 hasConceptScore W3030522011C3019054536 @default.
- W3030522011 hasConceptScore W3030522011C41008148 @default.
- W3030522011 hasConceptScore W3030522011C502942594 @default.
- W3030522011 hasConceptScore W3030522011C71924100 @default.
- W3030522011 hasConceptScore W3030522011C86803240 @default.
- W3030522011 hasConceptScore W3030522011C90559484 @default.
- W3030522011 hasConceptScore W3030522011C95444343 @default.
- W3030522011 hasIssue "03" @default.
- W3030522011 hasLocation W30305220111 @default.
- W3030522011 hasOpenAccess W3030522011 @default.
- W3030522011 hasPrimaryLocation W30305220111 @default.
- W3030522011 hasRelatedWork W1473888720 @default.
- W3030522011 hasRelatedWork W2210088078 @default.
- W3030522011 hasRelatedWork W2254797597 @default.
- W3030522011 hasRelatedWork W227558992 @default.
- W3030522011 hasRelatedWork W2356692165 @default.
- W3030522011 hasRelatedWork W2366978942 @default.
- W3030522011 hasRelatedWork W2396450610 @default.
- W3030522011 hasRelatedWork W2424702816 @default.
- W3030522011 hasRelatedWork W2588664323 @default.
- W3030522011 hasRelatedWork W3028925234 @default.
- W3030522011 hasRelatedWork W3029168053 @default.
- W3030522011 hasRelatedWork W3029339808 @default.
- W3030522011 hasRelatedWork W3029989727 @default.
- W3030522011 hasRelatedWork W3030090927 @default.
- W3030522011 hasRelatedWork W3030195231 @default.
- W3030522011 hasRelatedWork W3031768179 @default.
- W3030522011 hasRelatedWork W3031840767 @default.
- W3030522011 hasRelatedWork W3032673438 @default.
- W3030522011 hasRelatedWork W3029343930 @default.
- W3030522011 hasRelatedWork W3031500618 @default.
- W3030522011 hasVolume "42" @default.
- W3030522011 isParatext "false" @default.
- W3030522011 isRetracted "false" @default.
- W3030522011 magId "3030522011" @default.
- W3030522011 workType "article" @default.