Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030551376> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W3030551376 endingPage "450" @default.
- W3030551376 startingPage "446" @default.
- W3030551376 abstract "ObjectiveTo identify Mobiluncus species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing and to analyze antibiotic resistance genes and phylogenetic relationships among Mobiluncus species with whole genome sequencing.MethodsTwenty-five Mobiluncus strains were isolated after anaerobic culture of 65 vaginal secretion samples of patients with bacterial vaginosis (BV) and identified to species level by MALDI-TOF MS and 16S rRNA gene sequencing. The whole genome DNA of each strain was extracted for next-generation sequencing. SPAdes was used to assembly genomes. Resistance genes were searched in ARGD database. The core genome of all isolates was analyzed by Harvest to construct phylogenetic tree.ResultsMALDI-TOF MS could only identify Mobiluncus curtisii, while 16S rRNA gene sequencing could identify both Mobiluncus mulieris and Mobiluncus curtisii. Results of the whole genome sequencing showed that tetracycline resistance gene tet(o) and macrolides resistance gene erm(x) were the two predominant acquired resistance genes of Mobiluncus with a positive rate of 84.7% and 61.5% respectively. Intra-species relationships of the two Mobiluncus species were close, but a distant phylogenetic relationship was found between the two species.ConclusionThis study shows that MALDI-TOF MS can′t be used to identify Mobiluncus mulieris at present. Mobiluncus strains have potential resistance to tetracycline and macrolides. Intra-species evolution of Mobiluncus is slow, which indicates that there is no growing trend towards new species for the time being.Key words: Mobiluncus; Bacterial vaginosis (BV); MALDI-TOF MS; Whole genome sequencing; Resistance gene" @default.
- W3030551376 created "2020-06-05" @default.
- W3030551376 creator A5025221918 @default.
- W3030551376 creator A5026218314 @default.
- W3030551376 creator A5055273353 @default.
- W3030551376 creator A5060729880 @default.
- W3030551376 creator A5080042635 @default.
- W3030551376 creator A5080209629 @default.
- W3030551376 creator A5081855758 @default.
- W3030551376 creator A5083967189 @default.
- W3030551376 creator A5089795680 @default.
- W3030551376 date "2018-06-30" @default.
- W3030551376 modified "2023-09-26" @default.
- W3030551376 title "Classification and phylogenetic analysis of the genus Mobiluncus based on matrix-assisted laser de-sorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and whole genome sequen-cing" @default.
- W3030551376 doi "https://doi.org/10.3760/cma.j.issn.0254-5101.2018.06.008" @default.
- W3030551376 hasPublicationYear "2018" @default.
- W3030551376 type Work @default.
- W3030551376 sameAs 3030551376 @default.
- W3030551376 citedByCount "0" @default.
- W3030551376 crossrefType "journal-article" @default.
- W3030551376 hasAuthorship W3030551376A5025221918 @default.
- W3030551376 hasAuthorship W3030551376A5026218314 @default.
- W3030551376 hasAuthorship W3030551376A5055273353 @default.
- W3030551376 hasAuthorship W3030551376A5060729880 @default.
- W3030551376 hasAuthorship W3030551376A5080042635 @default.
- W3030551376 hasAuthorship W3030551376A5080209629 @default.
- W3030551376 hasAuthorship W3030551376A5081855758 @default.
- W3030551376 hasAuthorship W3030551376A5083967189 @default.
- W3030551376 hasAuthorship W3030551376A5089795680 @default.
- W3030551376 hasConcept C104317684 @default.
- W3030551376 hasConcept C141231307 @default.
- W3030551376 hasConcept C193252679 @default.
- W3030551376 hasConcept C42062724 @default.
- W3030551376 hasConcept C54355233 @default.
- W3030551376 hasConcept C86803240 @default.
- W3030551376 hasConcept C89423630 @default.
- W3030551376 hasConceptScore W3030551376C104317684 @default.
- W3030551376 hasConceptScore W3030551376C141231307 @default.
- W3030551376 hasConceptScore W3030551376C193252679 @default.
- W3030551376 hasConceptScore W3030551376C42062724 @default.
- W3030551376 hasConceptScore W3030551376C54355233 @default.
- W3030551376 hasConceptScore W3030551376C86803240 @default.
- W3030551376 hasConceptScore W3030551376C89423630 @default.
- W3030551376 hasIssue "6" @default.
- W3030551376 hasLocation W30305513761 @default.
- W3030551376 hasOpenAccess W3030551376 @default.
- W3030551376 hasPrimaryLocation W30305513761 @default.
- W3030551376 hasRelatedWork W1972535282 @default.
- W3030551376 hasRelatedWork W2018065226 @default.
- W3030551376 hasRelatedWork W2036398663 @default.
- W3030551376 hasRelatedWork W2070417538 @default.
- W3030551376 hasRelatedWork W2101034691 @default.
- W3030551376 hasRelatedWork W2132786899 @default.
- W3030551376 hasRelatedWork W2138260806 @default.
- W3030551376 hasRelatedWork W2164466789 @default.
- W3030551376 hasRelatedWork W2299151447 @default.
- W3030551376 hasRelatedWork W2405112344 @default.
- W3030551376 hasRelatedWork W2793016541 @default.
- W3030551376 hasRelatedWork W2888732443 @default.
- W3030551376 hasRelatedWork W2898220832 @default.
- W3030551376 hasRelatedWork W3006960114 @default.
- W3030551376 hasRelatedWork W3047036998 @default.
- W3030551376 hasRelatedWork W3125649320 @default.
- W3030551376 hasRelatedWork W3136068847 @default.
- W3030551376 hasRelatedWork W3212714800 @default.
- W3030551376 hasRelatedWork W2507430540 @default.
- W3030551376 hasRelatedWork W2761133658 @default.
- W3030551376 hasVolume "38" @default.
- W3030551376 isParatext "false" @default.
- W3030551376 isRetracted "false" @default.
- W3030551376 magId "3030551376" @default.
- W3030551376 workType "article" @default.