Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030594698> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W3030594698 endingPage "1558" @default.
- W3030594698 startingPage "1556" @default.
- W3030594698 abstract "Objective To investigate the effects of Kruppel-like factor 5 ( KLF5 ) on biological behaviors of RKO cell line.Methods With total RNA extracted from human intestinal cells as the template,KLF5 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with primers designed according to the sequence of GenBank,and the product was inserted into the pCDH-CMV-KLF5-EF1-copGFP to construct KLF5 lentivirus vector.RKO cells were divided into two groups:blank control group,KLF5 group. The synthesized KLF5 lentivirus vector was transfected into RKO cells. Real-time quantitative PCR and Western blotting were used to detect the KLF5 mRNA and protein levels.The proliferation of RKO cells was measured by cell counting Kit-8 (CCK-8) assay.The ability of motility and invasion of RKO cells was assessed by Transwell invasion assay.Results In the KLF5 group,the expression of KLF5 mRNA and protein was increased as compared with the blank control group ( P ＜ 0.05 ).The overexpression of KLF5 could significantly suppressed proliferation of RKO cells ( P ＜ 0.05 ).KLF5 could significantly inhibit the abilities of migration and invasion of RKO cells as compared with the blank control group ( 50.26 ± 2.17 vs 25.12 ± 2.27 ; 45.48 ± 1.53 vs 22.13 ± 2.25 ) (P＜0.05).Conclusion KLF5 could regulate the biological behaviors of RKO cell and be used as a target to prevent the colon cancer.Key words: Colon cancer; Lentivirus vector; Kruppel-like factor 5" @default.
- W3030594698 created "2020-06-05" @default.
- W3030594698 creator A5009880286 @default.
- W3030594698 creator A5013348879 @default.
- W3030594698 creator A5028395335 @default.
- W3030594698 creator A5043720480 @default.
- W3030594698 creator A5044932847 @default.
- W3030594698 creator A5063802914 @default.
- W3030594698 creator A5091798976 @default.
- W3030594698 date "2012-08-08" @default.
- W3030594698 modified "2023-09-28" @default.
- W3030594698 title "Effects of lentivirus vector-mediated Kruppel-like factor 5 transfection on proliferation and metastasis of RKO cells" @default.
- W3030594698 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2012.08.048" @default.
- W3030594698 hasPublicationYear "2012" @default.
- W3030594698 type Work @default.
- W3030594698 sameAs 3030594698 @default.
- W3030594698 citedByCount "0" @default.
- W3030594698 crossrefType "journal-article" @default.
- W3030594698 hasAuthorship W3030594698A5009880286 @default.
- W3030594698 hasAuthorship W3030594698A5013348879 @default.
- W3030594698 hasAuthorship W3030594698A5028395335 @default.
- W3030594698 hasAuthorship W3030594698A5043720480 @default.
- W3030594698 hasAuthorship W3030594698A5044932847 @default.
- W3030594698 hasAuthorship W3030594698A5063802914 @default.
- W3030594698 hasAuthorship W3030594698A5091798976 @default.
- W3030594698 hasConcept C104317684 @default.
- W3030594698 hasConcept C105580179 @default.
- W3030594698 hasConcept C11957725 @default.
- W3030594698 hasConcept C121608353 @default.
- W3030594698 hasConcept C1491633281 @default.
- W3030594698 hasConcept C153911025 @default.
- W3030594698 hasConcept C185592680 @default.
- W3030594698 hasConcept C203014093 @default.
- W3030594698 hasConcept C2522874641 @default.
- W3030594698 hasConcept C2779013556 @default.
- W3030594698 hasConcept C2779690655 @default.
- W3030594698 hasConcept C2780727368 @default.
- W3030594698 hasConcept C4692481 @default.
- W3030594698 hasConcept C502942594 @default.
- W3030594698 hasConcept C54009773 @default.
- W3030594698 hasConcept C54355233 @default.
- W3030594698 hasConcept C55493867 @default.
- W3030594698 hasConcept C62112901 @default.
- W3030594698 hasConcept C81885089 @default.
- W3030594698 hasConcept C85265743 @default.
- W3030594698 hasConcept C86803240 @default.
- W3030594698 hasConceptScore W3030594698C104317684 @default.
- W3030594698 hasConceptScore W3030594698C105580179 @default.
- W3030594698 hasConceptScore W3030594698C11957725 @default.
- W3030594698 hasConceptScore W3030594698C121608353 @default.
- W3030594698 hasConceptScore W3030594698C1491633281 @default.
- W3030594698 hasConceptScore W3030594698C153911025 @default.
- W3030594698 hasConceptScore W3030594698C185592680 @default.
- W3030594698 hasConceptScore W3030594698C203014093 @default.
- W3030594698 hasConceptScore W3030594698C2522874641 @default.
- W3030594698 hasConceptScore W3030594698C2779013556 @default.
- W3030594698 hasConceptScore W3030594698C2779690655 @default.
- W3030594698 hasConceptScore W3030594698C2780727368 @default.
- W3030594698 hasConceptScore W3030594698C4692481 @default.
- W3030594698 hasConceptScore W3030594698C502942594 @default.
- W3030594698 hasConceptScore W3030594698C54009773 @default.
- W3030594698 hasConceptScore W3030594698C54355233 @default.
- W3030594698 hasConceptScore W3030594698C55493867 @default.
- W3030594698 hasConceptScore W3030594698C62112901 @default.
- W3030594698 hasConceptScore W3030594698C81885089 @default.
- W3030594698 hasConceptScore W3030594698C85265743 @default.
- W3030594698 hasConceptScore W3030594698C86803240 @default.
- W3030594698 hasIssue "08" @default.
- W3030594698 hasLocation W30305946981 @default.
- W3030594698 hasOpenAccess W3030594698 @default.
- W3030594698 hasPrimaryLocation W30305946981 @default.
- W3030594698 hasRelatedWork W122097890 @default.
- W3030594698 hasRelatedWork W2066953160 @default.
- W3030594698 hasRelatedWork W2089964586 @default.
- W3030594698 hasRelatedWork W2149875489 @default.
- W3030594698 hasRelatedWork W2282515358 @default.
- W3030594698 hasRelatedWork W2370095162 @default.
- W3030594698 hasRelatedWork W2372606303 @default.
- W3030594698 hasRelatedWork W2383187978 @default.
- W3030594698 hasRelatedWork W2404457768 @default.
- W3030594698 hasRelatedWork W2943425547 @default.
- W3030594698 hasRelatedWork W3014911115 @default.
- W3030594698 hasRelatedWork W3028748972 @default.
- W3030594698 hasRelatedWork W3029213256 @default.
- W3030594698 hasRelatedWork W3030710407 @default.
- W3030594698 hasRelatedWork W3031300910 @default.
- W3030594698 hasRelatedWork W3031349113 @default.
- W3030594698 hasRelatedWork W3031886609 @default.
- W3030594698 hasRelatedWork W3031966358 @default.
- W3030594698 hasRelatedWork W3032099287 @default.
- W3030594698 hasRelatedWork W805298610 @default.
- W3030594698 hasVolume "29" @default.
- W3030594698 isParatext "false" @default.
- W3030594698 isRetracted "false" @default.
- W3030594698 magId "3030594698" @default.
- W3030594698 workType "article" @default.