Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030607452> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W3030607452 endingPage "1311" @default.
- W3030607452 startingPage "1308" @default.
- W3030607452 abstract "ObjectiveTo observe the effects of human leukocyte antigen (HLA)-F associated transcript 10 (FAT10)ΔGG, a carboxyl-terminal diglycine deficient mutant, on the proliferation and apoptosis of thyroid carcinoma cell line TPC-1.MethodsSpecimens of cervical carcinoma in situ and normal thyroid tissue, 10 each, were collected. The expression levels of FAT10 protein in these specimens were detected by Western blotting. Site-directed mutagenesis was applied to construct the mutant pcDNA3.0-flag-FAT10ΔGG plasmid. The TPC-1 cells were then transiently transfected with wild-type FAT10, FAT10ΔGG and empty vector (used as negative control), and the wild-type TPC-1 cells served as blank control group. The transfection efficiency of FAT10 or FAT10ΔGG was detected by Western blotting, and cell proliferation was determined by cell counting kit-8 (CCK-8) assay. Cisplatin was used to induce cells after cells were transfected for 24 h, and the cell apoptotic rate was examined by flow cytometry.ResultsWestern blotting showed a significantly increased expression of FAT10 protein in cervical carcinoma tissues as compared with that in normal cervical tissues. Over-expression of wild FAT10 in TPC-1 cells obviously promoted cell proliferation, but this promotion was significantly inhibited in cells transfected with its diglycine mutant. As compared with blank control group [(22.7±4.2)%] and negative control group [(24.1±3.8)%], the apoptotic rate was significantly reduced in wild FAT10 group [(10.9±2.0)%, P 0.05].ConclusionFAT10 can promote cell proliferation and inhibit cell apoptosis through its carboxyl-terminal diglycine motif, and it may play an essential role in carcinogenesis and development of cancer.Key words: Thyroid carcinoma; Human leukocyte antigen-F associated transcript 10; Apoptosis" @default.
- W3030607452 created "2020-06-05" @default.
- W3030607452 creator A5080176831 @default.
- W3030607452 date "2016-05-08" @default.
- W3030607452 modified "2023-09-22" @default.
- W3030607452 title "Effect of human leukocyte antigen-F associated transcript 10 on the proliferation and apoptosis of thyroid carcinoma cells" @default.
- W3030607452 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2016.05.041" @default.
- W3030607452 hasPublicationYear "2016" @default.
- W3030607452 type Work @default.
- W3030607452 sameAs 3030607452 @default.
- W3030607452 citedByCount "0" @default.
- W3030607452 crossrefType "journal-article" @default.
- W3030607452 hasAuthorship W3030607452A5080176831 @default.
- W3030607452 hasConcept C104317684 @default.
- W3030607452 hasConcept C1491633281 @default.
- W3030607452 hasConcept C153911025 @default.
- W3030607452 hasConcept C185592680 @default.
- W3030607452 hasConcept C190283241 @default.
- W3030607452 hasConcept C54009773 @default.
- W3030607452 hasConcept C54355233 @default.
- W3030607452 hasConcept C553184892 @default.
- W3030607452 hasConcept C55493867 @default.
- W3030607452 hasConcept C62112901 @default.
- W3030607452 hasConcept C81885089 @default.
- W3030607452 hasConcept C85265743 @default.
- W3030607452 hasConcept C86803240 @default.
- W3030607452 hasConceptScore W3030607452C104317684 @default.
- W3030607452 hasConceptScore W3030607452C1491633281 @default.
- W3030607452 hasConceptScore W3030607452C153911025 @default.
- W3030607452 hasConceptScore W3030607452C185592680 @default.
- W3030607452 hasConceptScore W3030607452C190283241 @default.
- W3030607452 hasConceptScore W3030607452C54009773 @default.
- W3030607452 hasConceptScore W3030607452C54355233 @default.
- W3030607452 hasConceptScore W3030607452C553184892 @default.
- W3030607452 hasConceptScore W3030607452C55493867 @default.
- W3030607452 hasConceptScore W3030607452C62112901 @default.
- W3030607452 hasConceptScore W3030607452C81885089 @default.
- W3030607452 hasConceptScore W3030607452C85265743 @default.
- W3030607452 hasConceptScore W3030607452C86803240 @default.
- W3030607452 hasIssue "5" @default.
- W3030607452 hasLocation W30306074521 @default.
- W3030607452 hasOpenAccess W3030607452 @default.
- W3030607452 hasPrimaryLocation W30306074521 @default.
- W3030607452 hasRelatedWork W2057656028 @default.
- W3030607452 hasRelatedWork W2262762789 @default.
- W3030607452 hasRelatedWork W2273578390 @default.
- W3030607452 hasRelatedWork W2360694950 @default.
- W3030607452 hasRelatedWork W2363479102 @default.
- W3030607452 hasRelatedWork W2369214776 @default.
- W3030607452 hasRelatedWork W2376695860 @default.
- W3030607452 hasRelatedWork W2401067645 @default.
- W3030607452 hasRelatedWork W2417034418 @default.
- W3030607452 hasRelatedWork W3026009584 @default.
- W3030607452 hasRelatedWork W3029497833 @default.
- W3030607452 hasRelatedWork W3029545767 @default.
- W3030607452 hasRelatedWork W3030275518 @default.
- W3030607452 hasRelatedWork W3030317359 @default.
- W3030607452 hasRelatedWork W3030622382 @default.
- W3030607452 hasRelatedWork W3031711274 @default.
- W3030607452 hasRelatedWork W3147208470 @default.
- W3030607452 hasRelatedWork W3165721909 @default.
- W3030607452 hasRelatedWork W2482595989 @default.
- W3030607452 hasRelatedWork W3197267127 @default.
- W3030607452 hasVolume "33" @default.
- W3030607452 isParatext "false" @default.
- W3030607452 isRetracted "false" @default.
- W3030607452 magId "3030607452" @default.
- W3030607452 workType "article" @default.