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- W3030617500 abstract "Objective To study the biocompatibility betwcen FKS06-chitosan membrane and cultured Schwann cells. Methods Purified and healthy third passage Schwann cells were co-cuhurod with chitosan film and FK506-chitosan film respectively, and with the cover glasses as control. Doubling time and the purity of the Schwann cells in the three groups were calculated. The growth status and curve of the Schwann ceils were measured by the MTr method. The cells were also stained with anti-S100 immunofluorescence. Results The doubling time of Sehwann cells cultured on cover glass, ehitosan film and FK506-chitosan film was 5.9 days, 4days and 4 days respectively. The purity was 80%, 89% and 93% respectively. Sehwann cells' proliferation state of the FKS06-ehitosan group was the best and that in the chitosan group was the next in order. The proliferation state in the cover glass group was the worst. The Schwann cells were S-100 positive, and the alignment of which grew on FK506-chitosan film was regular and showed whirlpool and palisade shape.Conclusion FK506-chitosan film possesses significantly better biocompatibility and bioactivity for Schwann cells and can maintain the biological characteristics of Schwann cells.Key words: Schwann cells; Peripheral nerves; FK506; Chitosan" @default.
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- W3030617500 date "2009-06-10" @default.
- W3030617500 modified "2023-09-27" @default.
- W3030617500 title "In vitro study of biocompatibility between FK506-chitosan film and cultured Schwann cells" @default.
- W3030617500 doi "https://doi.org/10.3760/cma.j.issn.1005-054x.2009.03.015" @default.
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