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- W3030637841 abstract "Objective To investigate the usefulness of a tissue engineering scaffold made from nano-silver and type-Ⅰ collagen in repair and regeneration of damaged peripheral nerves in adult rabbits.Methods Forty rabbits were randomly divided into an experimental group (nano-sliver-collagen-scaffold with laminin coatings) and a control group (collagen scaffold with laminin). The 10 mm defects of the rabbit sciatic nerves were repaired respectively in both groups. The functional recovery (regenerated nerve fibers and their myelination status) 30 days after surgery was examined by electrophysiology, Fluoro-Gold retrograde tracing,toluidine blue staining and transmission electron microscopy. Results Compared with the collagen-scaffold without nano-silver in the control group, the nano-silver-containing scaffold showed a higher rate of laminin adsorption, regenerated a nerve with a thicker myelin sheath and improved the nerve conduction velocity and nerve potential amplitude. Fluoro-Gold retrograde labeled the newly grown axons in the spinal cord cortex anterior horn and the dorsal root ganglion. Conclusion The nano-silver-collagen scaffold with laminin coating is effective in promoting axonal regeneration, with a recovery effect comparable to that of autologous nerve graft.Key words: Tissue engineering; Nanocomposites; Collagen; Gelatin; Laminin" @default.
- W3030637841 created "2020-06-05" @default.
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- W3030637841 date "2010-09-15" @default.
- W3030637841 modified "2023-09-26" @default.
- W3030637841 title "Repair and regeneration of damaged rabbit sciatic nerves with a tissue-engineered scaffold made from nano-silver and collagen type I" @default.
- W3030637841 doi "https://doi.org/10.3760/cma.j.issn.1671-7600.2010.09.012" @default.
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