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• W3030662008 abstract "ObjectiveTo discuss effects on the different storage temperature and time on glucose-6-phosphate dehydrogenase activity in dried blood spot specimens on filter paper. Select suitable storage conditions, so as to improve the accuracy of test results and reduce the false positive ratio.MethodsEach blood spot specimen was divided into 3 groups and separately stored at 3 different temperature, the fluorometric assay was used to detect glucose-6-phosphate dehydrogenase at the first, third and sixth day. Compared with the results of different groups and study the impact of temperature and time to the glucose-6-phosphate dehydrogenase activity.ResultsIn the 4℃ refrigeratory group, the glucose-6-phosphate dehydrogenase activity of 25 blood specimens detected by the fluorometric assay were normal, and the average result was(5. 32±0. 77)IU/g(Hb). There was a significant decline of glucose-6-phosphate dehydrogenase activity in blood spot specimens stored in the room temperature group(about 18 ℃)and the 37℃ incubator group than those in the 4℃ refrigeratory group(P<0. 01). Probable positive results appeared just at the third day in the room temperature group, but there was no probable positive results appeared in the 4℃ refrigeratory group during the sixth day. There were correlation between the glucose-6-phosphate dehydrogenase activity and storage temperature and time(R=0. 683,P<0.01), the glucose-6-phosphate dehydrogenase activity will be declined with the rising of storage temperature and the extending of storage time.ConclusionAlong with the rising of storage temperature and the prolonging of storage time, the glucose-6-phosphate dehydrogenase activity will be declined, and probable positive results even false positive results appeared. After natural drying of blood specimens in a horizontal position for at least 3 hours at room temperature, the most appropriate storage temperature for blood specimens is the 4℃ refrigerator. Blood specimens should avoid the prolonged exposure at room temperature or high-temperature environment.Key words: storage; temperature; time; glucose-6-phosphate dehydrogenase" @default.
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• W3030662008 date "2008-12-01" @default.
• W3030662008 modified "2023-09-29" @default.
• W3030662008 title "The Influence of Different Storage Temperature and Time on Glucose-6-Phosphate Dehydrogenase Activity in Dried Blood Spot Specimens on Filter Paper" @default.
• W3030662008 doi "https://doi.org/10.3877/cma.j.issn.1673-5250.2008.06.111" @default.
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