Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030685937> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W3030685937 endingPage "1320" @default.
- W3030685937 startingPage "1316" @default.
- W3030685937 abstract "Objective To established chronic intermittent hypoxic (CIH) animal model for investigate the mechanism of insulin resistance caused by CIH.Methods Twenty-four healthy male SD rats were randomly divided into four groups:normal control group (NC group),CIH 2 weeks group (CIH2 group),and CIH 5 weeks group (CIH5 group),with 8 rats in each group.The minimum oxygen concentration was 6％-7％ in animal chamber.About 8 hours per day (from 9AM to 5 PM),the experimental lasted for 35 days.The rats in control group were placed in the same animal box without CIH.After the experiment,to measure fasting blood glucose and insulin levels by radioimmunoassay and then according to the homeostasis model of insulin resistance index (HOMA) was calculated.The expressions of SOCS3 protein were detected with immunohistochemical method in rat liver cells.To detect the SOCS3-mRNA gene expression by reverse transcription and polymerase chain reaction (RT-PCR).Results Compared with the NC group,fasting blood glucose(F =33.582,P ＜0.05),insulin level (F =35.633,P ＜0.05) and HOMA-IR (F =49.045,P ＜0.05) of the CIH2.CIH5 group was higher than other groups (P ＜0.05).Compared with the NC group,the expression of SOCS3 protein was increased in CIH2 and CHI5 group (F =9.472,P ＜0.05),but in CIH2 and CIH5 group were no significantly differences (P ＞0.05).And then the expression of SOCS3-mRNA in CIH5 was higher than CIH2 and CIH5 group (F =8.665,P ＜ 0.05).Pearson correlation analysis showed that HOMA-IR and the expression of SOCS3 protein was negatively correlated (r =-0.759,P ＜0.001) and was positively correlated with SOCS3-mRNA (r =0.603,P =0.01).Conclusions ①CIH exposure caused fasting blood glucose level and increase insulin level and insulin resistance in rats with CIH.With the intermittent hypoxic exposure time prolonged,the degree of insulin resistance was increased.②CIH leads to increased expression of SOCS3 protein and SOCS3-mRNA gene in rat liver cells with CIH exposure,SOCS3 may be involved in the development of insulin resistance.Key words: Chronic intermittent hypoxia; SOCS3 ; Insulin resistance; Fluorescent quatititive PCR" @default.
- W3030685937 created "2020-06-05" @default.
- W3030685937 creator A5015791574 @default.
- W3030685937 creator A5034973373 @default.
- W3030685937 creator A5054264977 @default.
- W3030685937 date "2014-09-05" @default.
- W3030685937 modified "2023-09-27" @default.
- W3030685937 title "The effect of SOCS3 expression on rat liver cells by chronic intermittent hypoxic" @default.
- W3030685937 doi "https://doi.org/10.3760/cma.j.issn.1673-436x.2014.17.010" @default.
- W3030685937 hasPublicationYear "2014" @default.
- W3030685937 type Work @default.
- W3030685937 sameAs 3030685937 @default.
- W3030685937 citedByCount "0" @default.
- W3030685937 crossrefType "journal-article" @default.
- W3030685937 hasAuthorship W3030685937A5015791574 @default.
- W3030685937 hasAuthorship W3030685937A5034973373 @default.
- W3030685937 hasAuthorship W3030685937A5054264977 @default.
- W3030685937 hasConcept C104317684 @default.
- W3030685937 hasConcept C105580179 @default.
- W3030685937 hasConcept C121608353 @default.
- W3030685937 hasConcept C126042315 @default.
- W3030685937 hasConcept C126322002 @default.
- W3030685937 hasConcept C134018914 @default.
- W3030685937 hasConcept C176685330 @default.
- W3030685937 hasConcept C179185449 @default.
- W3030685937 hasConcept C2777391703 @default.
- W3030685937 hasConcept C2778117688 @default.
- W3030685937 hasConcept C2779306644 @default.
- W3030685937 hasConcept C4692481 @default.
- W3030685937 hasConcept C55493867 @default.
- W3030685937 hasConcept C63645605 @default.
- W3030685937 hasConcept C71924100 @default.
- W3030685937 hasConcept C86803240 @default.
- W3030685937 hasConceptScore W3030685937C104317684 @default.
- W3030685937 hasConceptScore W3030685937C105580179 @default.
- W3030685937 hasConceptScore W3030685937C121608353 @default.
- W3030685937 hasConceptScore W3030685937C126042315 @default.
- W3030685937 hasConceptScore W3030685937C126322002 @default.
- W3030685937 hasConceptScore W3030685937C134018914 @default.
- W3030685937 hasConceptScore W3030685937C176685330 @default.
- W3030685937 hasConceptScore W3030685937C179185449 @default.
- W3030685937 hasConceptScore W3030685937C2777391703 @default.
- W3030685937 hasConceptScore W3030685937C2778117688 @default.
- W3030685937 hasConceptScore W3030685937C2779306644 @default.
- W3030685937 hasConceptScore W3030685937C4692481 @default.
- W3030685937 hasConceptScore W3030685937C55493867 @default.
- W3030685937 hasConceptScore W3030685937C63645605 @default.
- W3030685937 hasConceptScore W3030685937C71924100 @default.
- W3030685937 hasConceptScore W3030685937C86803240 @default.
- W3030685937 hasIssue "17" @default.
- W3030685937 hasLocation W30306859371 @default.
- W3030685937 hasOpenAccess W3030685937 @default.
- W3030685937 hasPrimaryLocation W30306859371 @default.
- W3030685937 hasRelatedWork W2053864686 @default.
- W3030685937 hasRelatedWork W2239468360 @default.
- W3030685937 hasRelatedWork W2340355985 @default.
- W3030685937 hasRelatedWork W2413080893 @default.
- W3030685937 hasRelatedWork W2416780404 @default.
- W3030685937 hasRelatedWork W2417413071 @default.
- W3030685937 hasRelatedWork W2438359002 @default.
- W3030685937 hasRelatedWork W2461423593 @default.
- W3030685937 hasRelatedWork W2469807460 @default.
- W3030685937 hasRelatedWork W2473669512 @default.
- W3030685937 hasRelatedWork W2518497722 @default.
- W3030685937 hasRelatedWork W2580692147 @default.
- W3030685937 hasRelatedWork W2586200865 @default.
- W3030685937 hasRelatedWork W268406621 @default.
- W3030685937 hasRelatedWork W2913772802 @default.
- W3030685937 hasRelatedWork W3003076683 @default.
- W3030685937 hasRelatedWork W3008327033 @default.
- W3030685937 hasRelatedWork W3024640508 @default.
- W3030685937 hasRelatedWork W3159332893 @default.
- W3030685937 hasRelatedWork W575980832 @default.
- W3030685937 hasVolume "34" @default.
- W3030685937 isParatext "false" @default.
- W3030685937 isRetracted "false" @default.
- W3030685937 magId "3030685937" @default.
- W3030685937 workType "article" @default.