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- W3030688810 abstract "目的 对我国北方两地区小学生沙眼患者结膜样本进行聚合酶链反应(PCR)检测,并分析其病原流行株的基因类型.方法 按照世界卫生组织沙眼分期标准,在宁夏回族自治区银川市和河北省武强县两地采集临床诊断为沙眼的小学生结膜样本共213份.同时在两地分别采集正常小学生结膜样本作对照.利用PCR检测沙眼衣原体质粒片段,对质粒PCR检测阳性者通过套式PCR扩增沙眼衣原体主要外膜蛋白(MOMP)基因(omp-1)第4可变区片段,再经序列分析进行基因分型.结果 银川市45份样本中,质粒PCR检测阳性22份,检出率为48.9%;对17份样本进行套式PCR基因分型,显示B型为16份(94.1%),C型为1份(5.9%).武强县168份样本中,质粒PCR检测阳性109份,检出率为64.9%;对34份样本进行套式PCR基因分型,显示B型为28份(82.4%),C型为6份(17.6%).结论 沙眼在中国西北部银川市和华北部武强县的小学生中仍有流行,其流行株的基因类型以B型为主。" @default.
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- W3030688810 date "2007-06-15" @default.
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- W3030688810 title "Detection and genotyping of Chlamydia trachomatis in the primary school children of two districts of north China" @default.
- W3030688810 doi "https://doi.org/10.3760/cma.j.issn.2095-0160.2007.06.018" @default.
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