Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030691808> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3030691808 endingPage "52" @default.
- W3030691808 startingPage "49" @default.
- W3030691808 abstract "Objective To investigate the effect of insulin-like growth factor-1 (IGF-1 ) on the osteogenic phenotype of fibroblasts. Methods Fibroblasts (Fbs) were derived from an adult New Zealand white rabbit through isolation,purification and cultivation.The experiment was conducted in 3 groups.In the control group,Fbs were cultured in conventional medium without any intervention factors.In the osteogenic induction group,the osteogenic induction medium was composed of conventional medium plus dexamethasone at concentration of 1 × 10-8 mol/L plus vitamin C at 50 mg/L plus sodium glycerophosphate-β at 10 mmol/L.In the experimental group,Fbs were cultured in osteogenic induction medium plus IGF-1 at the final concentration of50 ng/mL.Proliferation of Fbs in each group was detected by methyl thiazolyl tetrazolium (MTT)colorimetric assay.Alkaline phosphatase (ALP) activiiy was detected,osteocalcin 6 days after culture was determined,and the ability of osteogenic differentiation 2 weeks after culture was evaluated by calcium-cobalt staining. Results MTT showed that the cells grew significantly faster in the experimental group than in the other 2 groups( P ＜ 0.05),but there was no significant difference between the control group and the osteogenic induction group in this respect (P ＞ 0.05).The ALP expression in the experimental group was insignificantly higher than in the osteogenic induction group( P ＜ 0.05),but significantly higher than in the control group ( P ＜ 0.05).In osteocalcin secretory activity,the experimental group was significantly superior to the other 2 groups and the osteogenic induction group was significantly superior to the control group ( P ＜0.05).The experimental group had significantly more calcified nodules than the other 2 groups and there were few calcified nodules in the control group 2 weeks after culture. Conclusion IGF-1 can advance proliferation and osteogenic phenotype expression of the Fb stimulated.Key words: Insulin-like growth factor-1; Fibroblasts; Cell proliferation" @default.
- W3030691808 created "2020-06-05" @default.
- W3030691808 creator A5003554962 @default.
- W3030691808 creator A5015887415 @default.
- W3030691808 creator A5044675667 @default.
- W3030691808 creator A5054054476 @default.
- W3030691808 creator A5089916595 @default.
- W3030691808 date "2012-01-15" @default.
- W3030691808 modified "2023-09-22" @default.
- W3030691808 title "The effect of insulin-like growth factor-1 on the osteogenic phenotvpe of fibroblasts" @default.
- W3030691808 doi "https://doi.org/10.3760/cma.j.issn.1671-7600.2012.01.013" @default.
- W3030691808 hasPublicationYear "2012" @default.
- W3030691808 type Work @default.
- W3030691808 sameAs 3030691808 @default.
- W3030691808 citedByCount "0" @default.
- W3030691808 crossrefType "journal-article" @default.
- W3030691808 hasAuthorship W3030691808A5003554962 @default.
- W3030691808 hasAuthorship W3030691808A5015887415 @default.
- W3030691808 hasAuthorship W3030691808A5044675667 @default.
- W3030691808 hasAuthorship W3030691808A5054054476 @default.
- W3030691808 hasAuthorship W3030691808A5089916595 @default.
- W3030691808 hasConcept C126322002 @default.
- W3030691808 hasConcept C131075544 @default.
- W3030691808 hasConcept C134018914 @default.
- W3030691808 hasConcept C142724271 @default.
- W3030691808 hasConcept C153911025 @default.
- W3030691808 hasConcept C160160445 @default.
- W3030691808 hasConcept C16685009 @default.
- W3030691808 hasConcept C170493617 @default.
- W3030691808 hasConcept C181199279 @default.
- W3030691808 hasConcept C185592680 @default.
- W3030691808 hasConcept C202751555 @default.
- W3030691808 hasConcept C2775960820 @default.
- W3030691808 hasConcept C2778260815 @default.
- W3030691808 hasConcept C2780689927 @default.
- W3030691808 hasConcept C2780783641 @default.
- W3030691808 hasConcept C55493867 @default.
- W3030691808 hasConcept C71924100 @default.
- W3030691808 hasConcept C74864618 @default.
- W3030691808 hasConcept C86803240 @default.
- W3030691808 hasConceptScore W3030691808C126322002 @default.
- W3030691808 hasConceptScore W3030691808C131075544 @default.
- W3030691808 hasConceptScore W3030691808C134018914 @default.
- W3030691808 hasConceptScore W3030691808C142724271 @default.
- W3030691808 hasConceptScore W3030691808C153911025 @default.
- W3030691808 hasConceptScore W3030691808C160160445 @default.
- W3030691808 hasConceptScore W3030691808C16685009 @default.
- W3030691808 hasConceptScore W3030691808C170493617 @default.
- W3030691808 hasConceptScore W3030691808C181199279 @default.
- W3030691808 hasConceptScore W3030691808C185592680 @default.
- W3030691808 hasConceptScore W3030691808C202751555 @default.
- W3030691808 hasConceptScore W3030691808C2775960820 @default.
- W3030691808 hasConceptScore W3030691808C2778260815 @default.
- W3030691808 hasConceptScore W3030691808C2780689927 @default.
- W3030691808 hasConceptScore W3030691808C2780783641 @default.
- W3030691808 hasConceptScore W3030691808C55493867 @default.
- W3030691808 hasConceptScore W3030691808C71924100 @default.
- W3030691808 hasConceptScore W3030691808C74864618 @default.
- W3030691808 hasConceptScore W3030691808C86803240 @default.
- W3030691808 hasIssue "01" @default.
- W3030691808 hasLocation W30306918081 @default.
- W3030691808 hasOpenAccess W3030691808 @default.
- W3030691808 hasPrimaryLocation W30306918081 @default.
- W3030691808 hasRelatedWork W1967320420 @default.
- W3030691808 hasRelatedWork W2356174745 @default.
- W3030691808 hasRelatedWork W2356319307 @default.
- W3030691808 hasRelatedWork W2356359199 @default.
- W3030691808 hasRelatedWork W2358397530 @default.
- W3030691808 hasRelatedWork W2359307081 @default.
- W3030691808 hasRelatedWork W2371479439 @default.
- W3030691808 hasRelatedWork W2371983824 @default.
- W3030691808 hasRelatedWork W2379684318 @default.
- W3030691808 hasRelatedWork W2379724710 @default.
- W3030691808 hasRelatedWork W2383039830 @default.
- W3030691808 hasRelatedWork W2386354554 @default.
- W3030691808 hasRelatedWork W2387637303 @default.
- W3030691808 hasRelatedWork W2467735196 @default.
- W3030691808 hasRelatedWork W3002604269 @default.
- W3030691808 hasRelatedWork W3025700728 @default.
- W3030691808 hasRelatedWork W3029484904 @default.
- W3030691808 hasRelatedWork W3031698145 @default.
- W3030691808 hasRelatedWork W3031719846 @default.
- W3030691808 hasRelatedWork W3122144494 @default.
- W3030691808 hasVolume "14" @default.
- W3030691808 isParatext "false" @default.
- W3030691808 isRetracted "false" @default.
- W3030691808 magId "3030691808" @default.
- W3030691808 workType "article" @default.