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• W3030702015 abstract "ObjectiveTo investigate the expression of transient receptor potential canonical channel-1 (TRPC1) in coronary smooth muscle cells (CSMCs) in diabetic rats and the possible mechanisms of TRPC1 channels on diabetic coronary function.MethodsA total of 160 healthy male Sprague-Dawley (SD) rats with 8-12 weeks of age and weight (200±20) g were randomly divided into normal control group (80 rats) and diabetic group (80 rats). Type 1 diabetes was established by intraperitoneally injection of streptozotocin. Normal and diabetic CSMCs from Sprague-Dawley rats were isolated by enzyme digestion. The protein and gene expressions of TRPC1 channels in normal and diabetic CSMCs were determined by Western blotting and real-time fluorescent quantitative PCR technique, respectively. Cytosolic calcium concentrations before and after incubating with TRPC1 channels blocker SKF96365 in normal and diabetic CSMCs were examined by recording the changes of fluorescence intensity ratios. Vascular reactivity after appliance of SKF96365 on coronary arteries in normal and diabetic rats was detected by using vascular tension measurement. The experimental data were analyzed by t test between two groups.ResultsThe protein expressions of TRPC1 channels in diabetic CSMCs were (1.456±0.081) times of the normal groups (t=-2.210, P<0.05). The gene expressions of TRPC1 channels in diabetic CSMCs were (2.198±0.251) times of the normal group(t=-3.864, P<0.05). ΔRatios of normal and diabetic CSMCs were 0.869±0.029 and (1.217±0.043, t=-6.644, P<0.05), respectively. ΔRatios were 0.842±0.020 and 0.067±0.039 before and after the appliance of TRPC1 channels blocker SKF96365 in normal CSMCs (t=15.726,P<0.05). ΔRatios were 1.217±0.043 and 0.195±0.028 before and after appliance of SKF96365 in diabetic CSMCs (t=-19.807, P<0.05). When the vascular tension increased to the maximum after application of ET-1, then coronary arteries were exposed to 10 μmol/L SKF96365, and the dilation rate of normal and diabetic coronary arteries were (69.2±6.0)% and (91.6±2.6)%, respectively (t=-3.529, P<0.05).ConclusionsThe expression of TRPC1 channels in diabetic CMSCs is increased, which increases cytosolic calcium concentrations, leading to vascular constriction and the dysfunction of diabetic coronary arteries. This may be one of the mechanisms that coronary heart diseases are commonly seen in diabetic patients.Key words: Transient receptor potential canonical channel-1; Diabetes mellitus; Coronary artery; Smooth muscle cells; Cytosolic calcium concentration" @default.
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• W3030702015 date "2017-05-27" @default.
• W3030702015 modified "2023-09-27" @default.
• W3030702015 title "The expression of classical transient receptor potential canonical channel-1 in coronary smooth muscle cells in diabetic rats and its effects on cytosolic calcium concentrations" @default.
• W3030702015 doi "https://doi.org/10.3760/cma.j.issn.1674-5809.2017.05.006" @default.
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