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- W3030758460 abstract "Objective To observe the antitumor immune effect of interleukin (IL)-7 gene for mouse mammary cancer treated with direct intratumoral injection.Methods IL-7 was constructed into the eukaryotic expression plasmid ( pcDNA3-IL-7 ) ; breast cancer cell TM40D BALB/C mice transplantation model was established ; direct intratumoral injection of IL-7 eukaryotic expression plasmid DNA was done;changes of tumor volume in mice were monitored; Enzyme linked immunosorbent assay (ELISA) assays were used to examine peripheral blood interferon (IFN)-γproduction in tumor-bearing mice; the expression levels of intracellular IFN-γ in tumor-bearing mice spleen cells were detected by using flow cytometry;local tumor after treatment underwent routine pathological analysis.Results IL-7 was successfully constructed into the eukaryotic expression plasmid ( pcDNA3-IL-7 ) ; the tumor growth rate in pcDNA3-IL-7 injection group was significantly reduced as compared with control group (P ＜ 0.05 ) ; the production of peripheral blood IFN-γ in pcDNA3-IL-7 injection group (242.3 ± 10.1 ) ng/L was significantly higher than in PBS group ( 115.2 ± 11.8) ng/L and pcDNA3 group ( 133.6 ± 9.4) ng/L; the secretion of intracellular IFN-γ in CD4 + T cells and CD8 + T cells was increased significantly; routine pathologic examination showed that there were significant histological changes ( a large number of visible tumor necrosis,plentiful inflammatory cells and large lymphocytes infiltration) in the mice with direct intratumoral injection of IL-7.Condusion Direct intratumoral injection of IL-7 gene eukaryotic expression plasmid significantly can inhibit the growth of mammary tumors in mice,significantly promote IFN-γ secretion in mice,and enhance the body' s immune response to mouse mammary tumor.Key words: Interleukin-7 ; Intedeukin-γ; Breast carcinoma; Antitumor immunity" @default.
- W3030758460 created "2020-06-05" @default.
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- W3030758460 date "2012-01-08" @default.
- W3030758460 modified "2023-09-23" @default.
- W3030758460 title "Antitumor immune effect of interleulin-7 gene for mouse mammary cancer treated with direct iatratumoral injection" @default.
- W3030758460 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2012.01.024" @default.
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