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- W3030763766 abstract "目的 明确新生小牛视网膜视杆-双极细胞体外发育过程中蛋白激酶Cα亚型(PKCα)的表达规律.方法分离培养新生小牛视网膜神经细胞,培养10、20、30和40 d的神经元用于免疫细胞化学染色,采用PKCα抗体标记视网膜视杆-双极细胞,用图像分析系统定量测量阳性细胞的免疫反应强度.结果培养10 d时,PKCα阳性神经元只有1～2个较短的神经突起;到培养20 d时,阳性细胞的一端为1个细长的神经突起,相对的另一端为丛状的短的树状突起,培养30 d及40 d的神经元保持培养20 d时神经元的形态特征.不同培养阶段的阳性细胞中PKCα免疫反应物质均位于细胞体和神经突起,定量分析显示培养20 d的阳性细胞免疫反应强度最高,培养30 d和40 d时阳性细胞PKCα免疫反应强度下降.结论培养20 d的视杆-双极细胞具备细胞形态和免疫反应特性上的成熟特征,PKCα的表达水平最高,为发育成熟的神经元.培养30 d以后,视杆-双极细胞逐渐衰老.实验结果为选择合适的供体材料进行视网膜移植提供了理论依据。" @default.
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- W3030763766 date "2005-12-15" @default.
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- W3030763766 title "Expression of protein kinase Cα in developing retinal rod-bipolar cells in vitro" @default.
- W3030763766 doi "https://doi.org/10.3760/cma.j.issn.2095-0160.2005.06.007" @default.
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