Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030764037> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W3030764037 endingPage "213" @default.
- W3030764037 startingPage "209" @default.
- W3030764037 abstract "ObjectiveTo evaluate the impact of production process change on the quality of varicella vaccine through analyzing the gene sequence of varicella-zoster Oka strain and the quality of varicella vaccine before and after application of cell factory in cell culture.MethodsThe viral DNA was extracted from bulk of varicella vaccine and fragments were amplified by PCR. The whole gene sequence of Oka strain was obtained by DNA sequencing with PCR products or recombinant plasmids and compared to the relevant sequences in GenBank. The virus titers, residual bovine serum albumin and residual antibiotics were compared between vaccines before and after production process change.ResultsThe genetic sequence of vaccines before and after production process change was consistent, which had typical characteristics of Oka strain. No new mutations were found. 33 single nucleotide polymorphism loci were found with a mixture of both parental and vaccine-type bases. The mean virus titers of vaccine before and after production process change were 5.00 and 5.02 lg plaque-forming unit/ml, respectively, and there was no statistical difference between them (t=1.1, P=0.26). The key quality indexes of the vaccine before and after the process change all met the enterprise standards and pharmacopoeia requirements.ConclusionAfter introducing the cell factory into the production process, the varicella vaccine has similar safety and quality consistency with the product prepared by original process.Key words: Chickenpox vaccine; Herpesvirus 3, human; Sequence analysis; Oka strain" @default.
- W3030764037 created "2020-06-05" @default.
- W3030764037 creator A5002028745 @default.
- W3030764037 creator A5020701892 @default.
- W3030764037 creator A5046319369 @default.
- W3030764037 creator A5056099062 @default.
- W3030764037 creator A5072172819 @default.
- W3030764037 creator A5076240767 @default.
- W3030764037 creator A5078716858 @default.
- W3030764037 creator A5079465511 @default.
- W3030764037 creator A5079943584 @default.
- W3030764037 creator A5085737308 @default.
- W3030764037 date "2017-10-10" @default.
- W3030764037 modified "2023-09-28" @default.
- W3030764037 title "Analysis of Oka strain gene sequence and varicella vaccine quality before and after production process change" @default.
- W3030764037 doi "https://doi.org/10.3760/cma.j.issn.1673-4211.2017.05.001" @default.
- W3030764037 hasPublicationYear "2017" @default.
- W3030764037 type Work @default.
- W3030764037 sameAs 3030764037 @default.
- W3030764037 citedByCount "0" @default.
- W3030764037 crossrefType "journal-article" @default.
- W3030764037 hasAuthorship W3030764037A5002028745 @default.
- W3030764037 hasAuthorship W3030764037A5020701892 @default.
- W3030764037 hasAuthorship W3030764037A5046319369 @default.
- W3030764037 hasAuthorship W3030764037A5056099062 @default.
- W3030764037 hasAuthorship W3030764037A5072172819 @default.
- W3030764037 hasAuthorship W3030764037A5076240767 @default.
- W3030764037 hasAuthorship W3030764037A5078716858 @default.
- W3030764037 hasAuthorship W3030764037A5079465511 @default.
- W3030764037 hasAuthorship W3030764037A5079943584 @default.
- W3030764037 hasAuthorship W3030764037A5085737308 @default.
- W3030764037 hasConcept C104317684 @default.
- W3030764037 hasConcept C159047783 @default.
- W3030764037 hasConcept C159654299 @default.
- W3030764037 hasConcept C2522874641 @default.
- W3030764037 hasConcept C2780473352 @default.
- W3030764037 hasConcept C2780801004 @default.
- W3030764037 hasConcept C2908772859 @default.
- W3030764037 hasConcept C54355233 @default.
- W3030764037 hasConcept C54602769 @default.
- W3030764037 hasConcept C60987743 @default.
- W3030764037 hasConcept C86803240 @default.
- W3030764037 hasConceptScore W3030764037C104317684 @default.
- W3030764037 hasConceptScore W3030764037C159047783 @default.
- W3030764037 hasConceptScore W3030764037C159654299 @default.
- W3030764037 hasConceptScore W3030764037C2522874641 @default.
- W3030764037 hasConceptScore W3030764037C2780473352 @default.
- W3030764037 hasConceptScore W3030764037C2780801004 @default.
- W3030764037 hasConceptScore W3030764037C2908772859 @default.
- W3030764037 hasConceptScore W3030764037C54355233 @default.
- W3030764037 hasConceptScore W3030764037C54602769 @default.
- W3030764037 hasConceptScore W3030764037C60987743 @default.
- W3030764037 hasConceptScore W3030764037C86803240 @default.
- W3030764037 hasIssue "5" @default.
- W3030764037 hasLocation W30307640371 @default.
- W3030764037 hasOpenAccess W3030764037 @default.
- W3030764037 hasPrimaryLocation W30307640371 @default.
- W3030764037 hasRelatedWork W2014357708 @default.
- W3030764037 hasRelatedWork W2047976861 @default.
- W3030764037 hasRelatedWork W2136745997 @default.
- W3030764037 hasRelatedWork W2166943269 @default.
- W3030764037 hasRelatedWork W2292161023 @default.
- W3030764037 hasRelatedWork W2351484642 @default.
- W3030764037 hasRelatedWork W2357794774 @default.
- W3030764037 hasRelatedWork W2360478018 @default.
- W3030764037 hasRelatedWork W2419510429 @default.
- W3030764037 hasRelatedWork W3105866825 @default.
- W3030764037 hasRelatedWork W3133712374 @default.
- W3030764037 hasRelatedWork W3148302600 @default.
- W3030764037 hasRelatedWork W2733360635 @default.
- W3030764037 hasRelatedWork W2768696128 @default.
- W3030764037 hasRelatedWork W2777747667 @default.
- W3030764037 hasRelatedWork W2840305879 @default.
- W3030764037 hasRelatedWork W2871634364 @default.
- W3030764037 hasRelatedWork W2881392567 @default.
- W3030764037 hasRelatedWork W3017535195 @default.
- W3030764037 hasRelatedWork W3177941184 @default.
- W3030764037 hasVolume "40" @default.
- W3030764037 isParatext "false" @default.
- W3030764037 isRetracted "false" @default.
- W3030764037 magId "3030764037" @default.
- W3030764037 workType "article" @default.