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- W3030845902 abstract "目的 分析人胃癌细胞系HGC-27细胞中肿瘤干细胞相关的SP(side population)亚群比例,分选出相关的SP和NSP细胞,并鉴别相关的SP是否具有干细胞某些生物学特性.方法 制备人胃癌细胞系HGC-27细胞中细胞悬液,经Hoechst33342和PI染色(其中对照组同时加入拮抗剂维拉帕米),流式细胞仪分析相关SP亚群.通过流式细胞仪分选出人胃癌细胞系HGC-27细胞中相关的SP细胞.克隆形成实验和MTT实验检测相关的SP和NSP细胞增殖能力差异.结果 SP亚群占HGC-27细胞中的6.00%左右,维拉帕米阻断后减少为0.01%左右.流式细胞仪分选出SP细胞8.5×105个,大部分为NSP细胞.克隆形成实验和MTT实验证实相关的SP细胞和NSP细胞增殖能力有差异.结论 人胃癌细胞系HGC-27细胞中存在相关的SP亚群细胞,维拉帕米可抑制染料外排而明显减少SP细胞的比例.可以通过流式细胞仪分选出相关的SP细胞,通过克隆形成实验和MTT实验检测相关的SP和NSP细胞增殖能力有差异.但尚需从分子水平证实胃癌细胞SP细胞相关干细胞特异性。" @default.
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- W3030845902 date "2013-12-15" @default.
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- W3030845902 title "Study on side population in human gastric cancer cell line HGC-27" @default.
- W3030845902 doi "https://doi.org/10.3760/cma.j.issn.1008-6706.2013.24.001" @default.
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