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- W3030877002 abstract "Objective To explore the effect of ischemia reperfusion-injured (IRI) kidney homogenate in vitro on CXC chemokine receptor 4 (CXCR4) expression in bone mesenchymal stem cells (BMSCs) and renal protective growth factors paracrine.Methods The renal homogenate supernatant of IRI model was built and co-cultured with BMSCs.CXCR4 protein expression was detected by laser focal microscope,chemotactic ability of BMSCs to stromal cells derived factor-1α (SDF-1α) was investigated by Transwell test,and the mRNA expression of SDF-1α,CXCR4 and hepatocyte growth factor (HGF) was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results CXCR4 protein expression was increased significantly after co-culture of BMSCs with IRI homogenate [2.54 ± 0.22 vs.1.26 ± 0.18,P ＜0.01),which ould be decreased by CXCR4 neutralizing antibody,and the chemotactic ability of BMSCs to SDF-1 increased at the same time [(26.72 ±5.61),(30.44 ±6.03),(38.92 ±6.79)/cm2 vs.(18.47 ±5.02)/cm2,P ＜0.01].SDF-1α mRNA expression was increased in IRI group,but had no significan difference among groups (P ＞ 0.05).CXCR4 mRNA (3.11 ± 0.24 vs.2.02 ± 0.17,P ＜ 0.01) and HGF mRNA (3.01 ±0.46 vs.1.99 ±0.21,P ＜0.01) expression was increased significantly with the homogenate,and the expression could be restrained by CXCR4 neutrlizing antibody (1.86 ± 0.11 and 1.78 ± 0.22,P ＜0.05).Conclusion IRI kidney microenvironment can promote chemotactic ability of BMSCs by increasing BMSCs surface CXCR4 expression,and then CXCR4 + BMSCs possessed the paracrine capabilities which promoted the recovery of the injured kidney.Key words: Renal ischemia; Reperfusion injury; Bone mesenchymal stem cells; CXC chemokine receptor 4" @default.
- W3030877002 created "2020-06-05" @default.
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- W3030877002 date "2014-12-08" @default.
- W3030877002 modified "2023-09-24" @default.
- W3030877002 title "Effect of ischemia reperfusion-injured kidney microenvironment on CXC chemokine receptor 4 expression in bone mesenchymal stem cells in vitro" @default.
- W3030877002 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2014.12.018" @default.
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