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- W3030887749 abstract "ObjectiveTo develop and evaluate a fluorescent antibody to membrane antigen (FAMA) test for detection of varicella-zoster virus(VZV)-specific antibody in plasma.MethodsThe FAMA test was established with VZV-infected cells as fixed antigen and fluorescein isothiocyanate labeled goat anti-human IgG as secondary antibody. The sensitivity, specificity and repeatability of the technique were validated. The antibodies in 200 plasma samples were determined with the established test and a commercial ELISA kit. The detection results of two methods were compared using Spearman’s rank correlation test.ResultsFAMA test had high sensitivity with the detecting limit of 0.04 IU/ml. No cross reaction with other herpesviruses, such as herpes simplex virus (HSV) 1, 2 and human cytomegalovirus was observed. The antibody positive rate and geometric mean titer of 200 samples determined by FAMA test were 93.5% and 1∶18.1, respectively. The result coincidence rate of FAMA test with the ELISA kit was 90.0%.The results of two methods were correlated with Spearman’s rank correlation coefficient 0.49, P<0.000 1.ConclusionThe developed method has good sensitivity, specificity and repeatability, and can be applied for detecting anti-VZV antibodies in sera.Key words: Fluorescent antibody technique; Herpesvirus 3, human; Immunoglobulin G" @default.
- W3030887749 created "2020-06-05" @default.
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- W3030887749 date "2018-06-10" @default.
- W3030887749 modified "2023-09-27" @default.
- W3030887749 title "Establishment and evaluation of a fluorescent antibody to membrane antigen test for varicella-zoster virus" @default.
- W3030887749 doi "https://doi.org/10.3760/cma.j.issn.1673-4211.2018.03.006" @default.
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