Matches in SemOpenAlex for { <https://semopenalex.org/work/W3030920722> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W3030920722 endingPage "240" @default.
- W3030920722 startingPage "236" @default.
- W3030920722 abstract "ObjectiveTo investigate the effect and molecular mechanism of Thioredoxin (Trx) on NADPH oxidase isoform Nox2 in human vascular endothelial cells in Atherosclerosis.MethodsAdenovirus vector gene transfer technology was used in human umbilical vein endothelial cells (HUVECs) to establish the cell model of Trx-overexpression cells (Ad-Trx) and its control cells (Ad-GFP). Oxidized low density lipoprotein (ox-LDL), a risk factor of atherosclerosis, was used as stimulator.Western Blot was used to detect the protein expression level of Nox2, Rac1 and Akt phosphorylation.Western Blot and immunofluorescence were used to detect Trx protein level in endothelial cells.DCFH-DA was used to detect intracellular reactive oxygen species (ROS) production.Extracting the cell membrane fractions, NADPH oxidase activity was measured by enzymatic method.ResultsWestern blot analysis showed adenoviral infection in endothelial cells successfully raised the expression of Trx, (by 3.3-fold, P<0.01) compare with the Ad-GFP cells.Trx overexpression significantly reduced ox-LDL-induced Nox2, Rac1 and p-Akt expression (down regulated 31.5%, 23.8% and 20.8%, respectively, P<0.05), suppressed the enhancements of ROS production (25.3%, P=0.0247) and NADPH oxidase activity (32.6%, P=0.004) in ox-LDL stimulated endothelial cells.ConclusionsTrx overexpression inhibited ox-LDL-induced Nox2 pathway excessive activation and oxidative stress by down regulating endothelial Nox2, Nox2 binding protein Rac1 expression and Nox2 downstream signaling molecule Akt phosphorylation, reduced inflammation damage and protected endothelial cell function.Key words: Atherosclerosis; Thioredoxin; Nox2; Rac1; p-Akt; Vascular endothelial cells" @default.
- W3030920722 created "2020-06-05" @default.
- W3030920722 creator A5009977037 @default.
- W3030920722 creator A5057603195 @default.
- W3030920722 creator A5060928832 @default.
- W3030920722 creator A5061184556 @default.
- W3030920722 creator A5078988597 @default.
- W3030920722 date "2016-06-25" @default.
- W3030920722 modified "2023-09-23" @default.
- W3030920722 title "Thioredoxin regulating Nox2 pathway in vascular endothelial cells" @default.
- W3030920722 doi "https://doi.org/10.3969/cma.j.issn.1007-5410.2016.03.014" @default.
- W3030920722 hasPublicationYear "2016" @default.
- W3030920722 type Work @default.
- W3030920722 sameAs 3030920722 @default.
- W3030920722 citedByCount "0" @default.
- W3030920722 crossrefType "journal-article" @default.
- W3030920722 hasAuthorship W3030920722A5009977037 @default.
- W3030920722 hasAuthorship W3030920722A5057603195 @default.
- W3030920722 hasAuthorship W3030920722A5060928832 @default.
- W3030920722 hasAuthorship W3030920722A5061184556 @default.
- W3030920722 hasAuthorship W3030920722A5078988597 @default.
- W3030920722 hasConcept C104317684 @default.
- W3030920722 hasConcept C11960822 @default.
- W3030920722 hasConcept C123012128 @default.
- W3030920722 hasConcept C153911025 @default.
- W3030920722 hasConcept C185592680 @default.
- W3030920722 hasConcept C202751555 @default.
- W3030920722 hasConcept C2776151105 @default.
- W3030920722 hasConcept C2776415932 @default.
- W3030920722 hasConcept C2779719074 @default.
- W3030920722 hasConcept C3623737 @default.
- W3030920722 hasConcept C48349386 @default.
- W3030920722 hasConcept C55493867 @default.
- W3030920722 hasConcept C75217442 @default.
- W3030920722 hasConcept C86803240 @default.
- W3030920722 hasConcept C95444343 @default.
- W3030920722 hasConceptScore W3030920722C104317684 @default.
- W3030920722 hasConceptScore W3030920722C11960822 @default.
- W3030920722 hasConceptScore W3030920722C123012128 @default.
- W3030920722 hasConceptScore W3030920722C153911025 @default.
- W3030920722 hasConceptScore W3030920722C185592680 @default.
- W3030920722 hasConceptScore W3030920722C202751555 @default.
- W3030920722 hasConceptScore W3030920722C2776151105 @default.
- W3030920722 hasConceptScore W3030920722C2776415932 @default.
- W3030920722 hasConceptScore W3030920722C2779719074 @default.
- W3030920722 hasConceptScore W3030920722C3623737 @default.
- W3030920722 hasConceptScore W3030920722C48349386 @default.
- W3030920722 hasConceptScore W3030920722C55493867 @default.
- W3030920722 hasConceptScore W3030920722C75217442 @default.
- W3030920722 hasConceptScore W3030920722C86803240 @default.
- W3030920722 hasConceptScore W3030920722C95444343 @default.
- W3030920722 hasIssue "3" @default.
- W3030920722 hasLocation W30309207221 @default.
- W3030920722 hasOpenAccess W3030920722 @default.
- W3030920722 hasPrimaryLocation W30309207221 @default.
- W3030920722 hasRelatedWork W1974846409 @default.
- W3030920722 hasRelatedWork W1986841539 @default.
- W3030920722 hasRelatedWork W1986867700 @default.
- W3030920722 hasRelatedWork W2001278744 @default.
- W3030920722 hasRelatedWork W2020328349 @default.
- W3030920722 hasRelatedWork W2027075460 @default.
- W3030920722 hasRelatedWork W2027762258 @default.
- W3030920722 hasRelatedWork W2031230280 @default.
- W3030920722 hasRelatedWork W2071345457 @default.
- W3030920722 hasRelatedWork W2102744645 @default.
- W3030920722 hasRelatedWork W2102910971 @default.
- W3030920722 hasRelatedWork W2155084220 @default.
- W3030920722 hasRelatedWork W2156263462 @default.
- W3030920722 hasRelatedWork W2349814470 @default.
- W3030920722 hasRelatedWork W2378703741 @default.
- W3030920722 hasRelatedWork W2561642378 @default.
- W3030920722 hasRelatedWork W2592770187 @default.
- W3030920722 hasRelatedWork W2911745951 @default.
- W3030920722 hasRelatedWork W3032543208 @default.
- W3030920722 hasRelatedWork W3092550576 @default.
- W3030920722 hasVolume "21" @default.
- W3030920722 isParatext "false" @default.
- W3030920722 isRetracted "false" @default.
- W3030920722 magId "3030920722" @default.
- W3030920722 workType "article" @default.