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- W3030962034 abstract "目的 研究四氧化二氮(N2O4)对小鼠骨髓细胞姐妹染色单体互换(SCE)频率变化的影响.方法ICR小鼠,体重20～25 g,随机分为对照组和模型组,每组5只.染毒模型制作方法:将动物置于军事医学科学院提供的120 L染毒柜中,使用100 μl微量注射器定量抽取液态N2O4,注入120 L染毒柜中.染毒柜内备微型电扇,以保证推进剂充分蒸发及均匀分布,维持染毒柜内N2O4浓度为70 g/m3,染毒时间15 min.SCE标本制备:小鼠饲养3个月后,将小鼠用乙醚麻醉,埋入5-溴脱氧尿核苷药片,24 h处死小鼠,于活杀之前腹腔按3μg/g体重注射秋水仙素液,制备骨髓的染色体标本.采用荧光+吉姆萨染剂分染法处理标本.SCE记数方法:先在中倍镜(20×)下选M2期细胞,再用油镜观察互换次数,凡在中间互换的记为2次;在末端互换记为1次.每只小鼠记数30个M2期细胞,结果以互换次数/细胞表示.与对照组小鼠比较SCE频率. 结果N2O4染毒组的SCE频率(2.58)明显高于对照组(1.80).结论结果提示N2O4中毒对DNA有一定的损伤作用,并影响遗传物质的稳定。" @default.
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- W3030962034 date "2005-09-15" @default.
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- W3030962034 title "Changes of sister chromatid exchanged frequency in bone marrow cell of mice intoxicated with nitrogen tetroxide" @default.
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