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- W3031026742 abstract "Objective To explore the advantages and disadvantages of three methods for clinical screening and diagnosis in Hepatitis C.Methods 71 cases of Hepatitis C patients for experimental group,while 63 negative samples excluding Hepatitis C as control group.Antibody Hepatitis C Virus (Anti-HCV) was detected by PCR-fluorescent probe,chemiluminesent microparticle immunoassay(CMIA) and enzyme linked immunosorbent assay(ELISA) respectively.Results In the positive group,the sensitivity of PCR-fluorescent probe was 87.32％,CMIA was 100％,ELISA was 97.18％; Analyzed by chi-square test,x2=4.95,the difference between PCR-fluorescent probe and other kinds of methods was statistically significant (P ＜0.05),while no significant difference between CMIA and ELISA (P＞ 0.05).In control group,the specificity of PCR-fluorescent probe was 100％,CMIA 95.23％,ELISA 98.41％,respectively; Analyzed by x2test,the specificity of three methods had no statistically significant (P＞ 0.05),x2=3.84.Conclusions There were advantages and disadvantages for three methods in the diagnosis of Hepatitis C.One of the methods in CMIA and ELISA combined with PCR-fluorescence probe was recommended for diagnosing Hepatitis C.If you are going to monitor the treatment effect,PCR-fluorescence probe must be used; However,ELISA is widely used in most of the hospitals for the economic reason.When the result of Anti-HCV was suspicious or positive,PCR was needed to be detected to conform its authenticity.Key words: Hepatitis C Virus; Anti-HCV; PCR; CMIA; ELISA" @default.
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- W3031026742 date "2014-10-01" @default.
- W3031026742 modified "2023-09-23" @default.
- W3031026742 title "Application of three kinds of methods of diagnosis in Hepatitis C" @default.
- W3031026742 doi "https://doi.org/10.3760/cma.j.issn.1007-1245.2014.19.033" @default.
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