FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031051908> ?p ?o ?g. }

• W3031051908 endingPage "e0234147" @default.
• W3031051908 startingPage "e0234147" @default.
• W3031051908 abstract "Conversion of cellular prion protein (PrPC) into the pathogenic isoform of prion protein (PrPSc) in neurons is one of the key pathophysiological events in prion diseases. However, the molecular mechanism of neurodegeneration in prion diseases has yet to be fully elucidated because of a lack of suitable experimental models for analyzing neuron-autonomous responses to prion infection. In the present study, we used neuron-enriched primary cultures of cortical and thalamic mouse neurons to analyze autonomous neuronal responses to prion infection. PrPSc levels in neurons increased over the time after prion infection; however, no obvious neuronal losses or neurite alterations were observed. Interestingly, a finer analysis of individual neurons co-stained with PrPSc and phosphorylated protein kinase RNA-activated-like endoplasmic reticulum (ER) kinase (p-PERK), the early cellular response of the PERK-eukaryotic initiation factor 2 (eIF2α) pathway, demonstrated a positive correlation between the number of PrPSc granular stains and p-PERK granular stains, in cortical neurons at 21 dpi. Although the phosphorylation of PERK was enhanced in prion-infected cortical neurons, there was no sign of subsequent translational repression of synaptic protein synthesis or activations of downstream unfolded protein response (UPR) in the PERK-eIF2α pathway. These results suggest that PrPSc production in neurons induces ER stress in a neuron-autonomous manner; however, it does not fully activate UPR in prion-infected neurons. Our findings provide insights into the autonomous neuronal responses to prion propagation and the involvement of neuron-non-autonomous factor(s) in the mechanisms of neurodegeneration in prion diseases." @default.
• W3031051908 created "2020-06-05" @default.
• W3031051908 creator A5013790579 @default.
• W3031051908 creator A5019395170 @default.
• W3031051908 creator A5029164270 @default.
• W3031051908 creator A5077277139 @default.
• W3031051908 creator A5077970965 @default.
• W3031051908 date "2020-06-01" @default.
• W3031051908 modified "2023-09-26" @default.
• W3031051908 title "Enhanced phosphorylation of PERK in primary cultured neurons as an autonomous neuronal response to prion infection" @default.
• W3031051908 cites W1530155692 @default.
• W3031051908 cites W1533604120 @default.
• W3031051908 cites W1558969742 @default.
• W3031051908 cites W1590689549 @default.
• W3031051908 cites W1591693496 @default.
• W3031051908 cites W1604221682 @default.
• W3031051908 cites W1685885669 @default.
• W3031051908 cites W1969192163 @default.
• W3031051908 cites W1969193254 @default.
• W3031051908 cites W1971063209 @default.
• W3031051908 cites W1976442454 @default.
• W3031051908 cites W1977789448 @default.
• W3031051908 cites W1979164049 @default.
• W3031051908 cites W1983166842 @default.
• W3031051908 cites W1990417679 @default.
• W3031051908 cites W1997604339 @default.
• W3031051908 cites W1997938990 @default.
• W3031051908 cites W1998038512 @default.
• W3031051908 cites W2000966016 @default.
• W3031051908 cites W2004349171 @default.
• W3031051908 cites W2004369117 @default.
• W3031051908 cites W2004658424 @default.
• W3031051908 cites W2009995856 @default.
• W3031051908 cites W2010375635 @default.
• W3031051908 cites W2011827928 @default.
• W3031051908 cites W2022680168 @default.
• W3031051908 cites W2028279477 @default.
• W3031051908 cites W2035006933 @default.
• W3031051908 cites W2036677133 @default.
• W3031051908 cites W2042978244 @default.
• W3031051908 cites W2048516415 @default.
• W3031051908 cites W2051661723 @default.
• W3031051908 cites W2054504521 @default.
• W3031051908 cites W2069924169 @default.
• W3031051908 cites W2077316341 @default.
• W3031051908 cites W2077784911 @default.
• W3031051908 cites W2083056059 @default.
• W3031051908 cites W2089198803 @default.
• W3031051908 cites W2090339308 @default.
• W3031051908 cites W2099144222 @default.
• W3031051908 cites W2100347985 @default.
• W3031051908 cites W2101445704 @default.
• W3031051908 cites W2106963470 @default.
• W3031051908 cites W2108272195 @default.
• W3031051908 cites W2120736516 @default.
• W3031051908 cites W2122284973 @default.
• W3031051908 cites W2127848114 @default.
• W3031051908 cites W2134008603 @default.
• W3031051908 cites W2144078529 @default.
• W3031051908 cites W2145199559 @default.
• W3031051908 cites W2150524206 @default.
• W3031051908 cites W2167500696 @default.
• W3031051908 cites W2167700795 @default.
• W3031051908 cites W2192080449 @default.
• W3031051908 cites W2324660492 @default.
• W3031051908 cites W2401819178 @default.
• W3031051908 cites W2406691442 @default.
• W3031051908 cites W2409774290 @default.
• W3031051908 cites W2417368392 @default.
• W3031051908 cites W2708579841 @default.
• W3031051908 cites W2768532493 @default.
• W3031051908 cites W2804748500 @default.
• W3031051908 cites W2886044850 @default.
• W3031051908 cites W2891577239 @default.
• W3031051908 cites W2910480910 @default.
• W3031051908 cites W2947673562 @default.
• W3031051908 cites W2999849152 @default.
• W3031051908 cites W4211121722 @default.
• W3031051908 cites W4251115564 @default.
• W3031051908 cites W63738526 @default.
• W3031051908 doi "https://doi.org/10.1371/journal.pone.0234147" @default.
• W3031051908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7263615" @default.
• W3031051908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32479530" @default.
• W3031051908 hasPublicationYear "2020" @default.
• W3031051908 type Work @default.
• W3031051908 sameAs 3031051908 @default.
• W3031051908 citedByCount "5" @default.
• W3031051908 countsByYear W30310519082021 @default.
• W3031051908 countsByYear W30310519082022 @default.
• W3031051908 countsByYear W30310519082023 @default.
• W3031051908 crossrefType "journal-article" @default.
• W3031051908 hasAuthorship W3031051908A5013790579 @default.
• W3031051908 hasAuthorship W3031051908A5019395170 @default.
• W3031051908 hasAuthorship W3031051908A5029164270 @default.
• W3031051908 hasAuthorship W3031051908A5077277139 @default.
• W3031051908 hasAuthorship W3031051908A5077970965 @default.
• W3031051908 hasBestOaLocation W30310519081 @default.
• W3031051908 hasConcept C104317684 @default.

• next