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- W3031095112 abstract "Objective To elucidate the expression of costimulatory molecule B7-1 and B7-2 in liver hypothermic irrigation and reperfusion model of rats and its significance.Methods Thirty rats were randomly divided into three groups:group A,hypothermic irrigation for 20 min; group B,hypothermic irrigation for 30 min; and group C,sham operation.The expression of B7-1 and B7-2 mRNA in the livers was detected by using real-time reverse transcription polymerase chain reaction (RT-PCR).Results The B7-1 and B7-2 expression levels were at very low levels in the liver tissues of group C (0.226 ± 0.053,and 0.339 ±0.049),but the mRNA expression levels of B7-1 and B7-2 were significantly enhanced in group A or group B (P＜0.01).In addition,both B7-1 and B7-2 levels were higher in group B than in group A (B7-1:0.475± 0.079 vs 0.367 ± 0.052 ; B7-2:0.666 ± 0.039 vs 0.595 ± 0.050 ; P ＜ 0.01).Conclusion Hypothermic irrigation and reperfusion may increase the immunogenticity of the liver by upregulating the costimulatory molecules B7-1 and B7-2.Key words: Liver; Hypothermic perfusion; B7 expression" @default.
- W3031095112 created "2020-06-05" @default.
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- W3031095112 date "2013-03-08" @default.
- W3031095112 modified "2023-09-24" @default.
- W3031095112 title "Improvement of liver hypothermic irrigation and reperfusion model and expression of B7 in rats" @default.
- W3031095112 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2013.03.030" @default.
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