FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031192446> ?p ?o ?g. }

• W3031192446 endingPage "4199" @default.
• W3031192446 startingPage "4179" @default.
• W3031192446 abstract "A critical hurdle associated with natural killer (NK) cell immunotherapies is inadequate infiltration and function in the solid tumor microenvironment. Well-controlled 3D culture systems could advance our understanding of the role of various biophysical and biochemical cues that impact NK cell migration in solid tumors. The objectives of this study were to establish a biomaterial which (i) supports NK cell migration and (ii) recapitulates features of the in vivo solid tumor microenvironment, to study NK infiltration and function in a 3D system. Using peptide-functionalized poly(ethylene glycol)-based hydrogels, the extent of NK-92 cell migration was observed to be largely dependent on the density of integrin binding sites and the presence of matrix metalloproteinase degradable sites. When lung cancer cells were encapsulated into the hydrogels to create tumor microenvironments, the extent of NK-92 cell migration and functional activity was dependent on the cancer cell type and duration of 3D culture. NK-92 cells showed greater migration into the models consisting of nonmetastatic A549 cells relative to metastatic H1299 cells, and reduced migration in both models when cancer cells were cultured for 7 days versus 1 day. In addition, the production of NK cell-related pro-inflammatory cytokines and chemokines was reduced in H1299 models relative to A549 models. These differences in NK-92 cell migration and cytokine/chemokine production corresponded to differences in the production of various immunomodulatory molecules by the different cancer cells, namely, the H1299 models showed increased stress ligand shedding and immunosuppressive cytokine production, particularly TGF-β. Indeed, inhibition of TGF-β receptor I in NK-92 cells restored their infiltration in H1299 models to levels similar to that in A549 models and increased overall infiltration in both models. Relative to conventional 2D cocultures, NK-92 cell mediated cytotoxicity was reduced in the 3D tumor models, suggesting the hydrogel serves to mimic some features of the biophysical barriers in in vivo tumor microenvironments. This study demonstrates the feasibility of a synthetic hydrogel system for investigating the biophysical and biochemical cues impacting NK cell infiltration and NK cell-cancer cell interactions in the solid tumor microenvironment." @default.
• W3031192446 created "2020-06-05" @default.
• W3031192446 creator A5028558981 @default.
• W3031192446 creator A5046276024 @default.
• W3031192446 creator A5056118369 @default.
• W3031192446 creator A5079828924 @default.
• W3031192446 creator A5091648553 @default.
• W3031192446 date "2020-05-26" @default.
• W3031192446 modified "2023-09-27" @default.
• W3031192446 title "Engineered Three-Dimensional Tumor Models to Study Natural Killer Cell Suppression" @default.
• W3031192446 cites W140814971 @default.
• W3031192446 cites W1495950501 @default.
• W3031192446 cites W1505936474 @default.
• W3031192446 cites W1560838147 @default.
• W3031192446 cites W1585471068 @default.
• W3031192446 cites W1950871050 @default.
• W3031192446 cites W1959298931 @default.
• W3031192446 cites W1964514079 @default.
• W3031192446 cites W1965798034 @default.
• W3031192446 cites W1967717472 @default.
• W3031192446 cites W1968918729 @default.
• W3031192446 cites W1969021168 @default.
• W3031192446 cites W1970148422 @default.
• W3031192446 cites W1970300303 @default.
• W3031192446 cites W1971026939 @default.
• W3031192446 cites W1975836982 @default.
• W3031192446 cites W1978185825 @default.
• W3031192446 cites W1983596418 @default.
• W3031192446 cites W1988224733 @default.
• W3031192446 cites W1994261231 @default.
• W3031192446 cites W1998994531 @default.
• W3031192446 cites W2001244335 @default.
• W3031192446 cites W2008641024 @default.
• W3031192446 cites W2010866728 @default.
• W3031192446 cites W2011627863 @default.
• W3031192446 cites W2013224779 @default.
• W3031192446 cites W2023375384 @default.
• W3031192446 cites W2026780757 @default.
• W3031192446 cites W2028200036 @default.
• W3031192446 cites W2031938767 @default.
• W3031192446 cites W2032114357 @default.
• W3031192446 cites W2035538295 @default.
• W3031192446 cites W2038408021 @default.
• W3031192446 cites W2051190668 @default.
• W3031192446 cites W2052327379 @default.
• W3031192446 cites W2061305672 @default.
• W3031192446 cites W2061385235 @default.
• W3031192446 cites W2062133163 @default.
• W3031192446 cites W2062489242 @default.
• W3031192446 cites W2064501854 @default.
• W3031192446 cites W2064920472 @default.
• W3031192446 cites W2073647827 @default.
• W3031192446 cites W2074183516 @default.
• W3031192446 cites W2075521813 @default.
• W3031192446 cites W2078586977 @default.
• W3031192446 cites W2078608834 @default.
• W3031192446 cites W2081922256 @default.
• W3031192446 cites W2082305396 @default.
• W3031192446 cites W2083148270 @default.
• W3031192446 cites W2083289662 @default.
• W3031192446 cites W2087431312 @default.
• W3031192446 cites W2088807989 @default.
• W3031192446 cites W2090858884 @default.
• W3031192446 cites W2092215629 @default.
• W3031192446 cites W2095020121 @default.
• W3031192446 cites W2111688331 @default.
• W3031192446 cites W2113095561 @default.
• W3031192446 cites W2113542991 @default.
• W3031192446 cites W2114286636 @default.
• W3031192446 cites W2123143632 @default.
• W3031192446 cites W2124836729 @default.
• W3031192446 cites W2126240154 @default.
• W3031192446 cites W2130892977 @default.
• W3031192446 cites W2137695731 @default.
• W3031192446 cites W2141436376 @default.
• W3031192446 cites W2142041258 @default.
• W3031192446 cites W2143171862 @default.
• W3031192446 cites W2146494327 @default.
• W3031192446 cites W2148861635 @default.
• W3031192446 cites W2150932549 @default.
• W3031192446 cites W2152095288 @default.
• W3031192446 cites W2155859877 @default.
• W3031192446 cites W2156174107 @default.
• W3031192446 cites W2161229244 @default.
• W3031192446 cites W2165813814 @default.
• W3031192446 cites W2167279371 @default.
• W3031192446 cites W2205615606 @default.
• W3031192446 cites W2208179253 @default.
• W3031192446 cites W2284613379 @default.
• W3031192446 cites W2288113139 @default.
• W3031192446 cites W2289880980 @default.
• W3031192446 cites W2304593036 @default.
• W3031192446 cites W2334182897 @default.
• W3031192446 cites W2339885297 @default.
• W3031192446 cites W2347195823 @default.
• W3031192446 cites W2515812752 @default.
• W3031192446 cites W2558603479 @default.
• W3031192446 cites W2600487169 @default.

• next