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- W3031225229 abstract "ObjectiveTo confirm the role of podoplanin (PDPN) over-expressing human mammary fibroblasts (HMF) in tumorigenesis of breast cancer cell line MDA-MB-231, and study the effect and mechanism of reducing the peripheral platelet count in the progress of PDPN over-expressing HMF (HMF-P)-induced tumor growth.MethodsWe firstly constructed PDPN eukaryotic expression plasmid PDPN-pEGFP-LV5 and established HMF-P by lentivirus transfection. Then we investigated the effect of HMF-P on tumorigenesis in nude mice. We constructed a mouse model of thrombocytopenia by antibodies for mouse platelet depletion polyconal purified R300 and investigated the effect of HMF-P again in these special mice. Lastly, the expression of some tumor growth factors was detected by Western blotting.ResultsMDA-MB-231 cells commixed with HMF-P generated tumors of greatest volume 3.16 cm3; while the volume of HMF+ MDA-MB-231 and MDA-MB-231 groups was 1.93 cm3 and 0.52 cm3 respectively. The tumors volume in HMF-P + MDA-MB-231 group decreased to 1.03 cm3 in mice with thrombocytopenia, while the change in HMF+ MDA-MB-231 groups was not obvious. Western blotting analysis showed that the expression of platelet-derived growth factor-D (PDGF-D) in HMF-P + MDA-MB-231 group was significantly increased, and that in thrombocytopenia group was significantly decreased.ConclusionHMF-P can promote the tumorigenesis of MDA-MB-231. Its mechanism may be related to the interaction of PDPN with infiltrating platelets to promote its activation and release of PDGF-D. Reducing the peripheral blood platelet count to reduce platelet infiltration can inhibit the effect of HMF-P promoting tumor growth.Key words: Breast cancer; Platelets; Podoplanin; Mammary fibroblasts" @default.
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- W3031225229 date "2018-06-08" @default.
- W3031225229 modified "2023-09-28" @default.
- W3031225229 title "The roles and mechanism of platelets with podoplanin over-expressing human mammary fibroblasts in the in situ growth of breast cancer" @default.
- W3031225229 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2018.06.005" @default.
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