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- W3031243763 abstract "ObjectiveTo study the effect of lentiviral vector pSin-mediated gene modification on pluripotent stem cell lines.MethodsThe lentivirus pSin-GFP was packaged, iPS cells were divided into experimental group (cell and virus titer ratio 1 : 200) and control group (cell and virus titer ratio 1 : 1) and infected with virus. Stem cells were harvested and the single cell clones were obtained by limit dilution method. The cell clones after infection was calculated by subculture and pluripotent stem cell markers were detected by Western blot and immunofluorescence staining. The results were analyzed by variance analysis and t test.ResultsTwenty-four monoclonal cell lines were selected after 10 generations. Cell cloning number of the experimental group (4.67±0.33) was significantly fewer than that of the control group (16.00±0.58) (t =17.00, P < 0.01) . Stable passaged cells expressed Oct-4, Sox2, Nanog, SSEA-1 and Tra-1-60.ConclusionAfter genetic modification, the proliferation potential of iPS decreases, but their morphology is not changed and they express common stem cell markers.Key words: Pluripotent stem cells; Lentivirus; Genetically modified" @default.
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- W3031243763 date "2017-06-01" @default.
- W3031243763 modified "2023-09-23" @default.
- W3031243763 title "Effect of high dose pSin lentiviral vector-mediated transfection on pluripotent stem cells" @default.
- W3031243763 doi "https://doi.org/10.3877/cma.j.issn.2095-1221.2017.03.001" @default.
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