Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031298277> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W3031298277 endingPage "1641" @default.
- W3031298277 startingPage "1637" @default.
- W3031298277 abstract "ObjectiveTo investigate the basic biological functions of NKX3.1 with construction of a series of eukaryotic expression vector of NKX3.1 to study its effects on cell cycle and apoptosis.MethodsThe full-length reading frames (ORF) of NKX3.1 was amplified from normal human prostate tissue with polymerase chain reaction (PCR), the ORF was cloned into T vector, and then NKX3.1 was cloned into eukaryotic expression vector pcDNA3.1(+ ) and pcDNA3.1(-) with this T vector as template. NKX3.1 in pEGFP C1 was transfected into PC3 cell line, and C1 pEFP was taken as control to observe the localization of NKX3.1 in cells. At the same time, NKX3.1 in pcDNA3.1 and pcDNA3.1 were transfected into PC3 cells and the cells were collected after 24~48 hours to detect cell cycle and apoptosis by flow cytometry.ResultsThe eukaryotic expression vector of NKX3.1 was constructed successfully. Fluorescent protein was ocated in the nucleus after transfection of NKX3.1 in pEGFP C1, but pEGP C1 was both expressed in nucleus and cytoplasm. There was significant effect on the early apoptosis (P 0.05) after transfection of NKX3.1 compared to PC3 cell-transfected with empty vector of pcDNA3.1.ConclusionsThe result data suggest that NKX3.1 be nuclear protein and have effect on the early apoptosis of PC3 cell line based on the construction of eukaryotic expression vector.Key words: Genes, tumor suppressor; Genes, homeobox; Genetic vectors; Transfection; Prostatic neoplasms; Cell cycle; Apoptosis" @default.
- W3031298277 created "2020-06-05" @default.
- W3031298277 creator A5004004656 @default.
- W3031298277 creator A5008339460 @default.
- W3031298277 creator A5013736647 @default.
- W3031298277 creator A5019388488 @default.
- W3031298277 creator A5042543197 @default.
- W3031298277 creator A5078013001 @default.
- W3031298277 date "2016-11-20" @default.
- W3031298277 modified "2023-09-23" @default.
- W3031298277 title "The construction of human prostate specific homeobox gene NKX3.1 eukaryotic expression vector and preliminary study on the biological function of NKX3.1" @default.
- W3031298277 doi "https://doi.org/10.3760/cma.j.issn.1008-1372.2016.11.011" @default.
- W3031298277 hasPublicationYear "2016" @default.
- W3031298277 type Work @default.
- W3031298277 sameAs 3031298277 @default.
- W3031298277 citedByCount "0" @default.
- W3031298277 crossrefType "journal-article" @default.
- W3031298277 hasAuthorship W3031298277A5004004656 @default.
- W3031298277 hasAuthorship W3031298277A5008339460 @default.
- W3031298277 hasAuthorship W3031298277A5013736647 @default.
- W3031298277 hasAuthorship W3031298277A5019388488 @default.
- W3031298277 hasAuthorship W3031298277A5042543197 @default.
- W3031298277 hasAuthorship W3031298277A5078013001 @default.
- W3031298277 hasConcept C104317684 @default.
- W3031298277 hasConcept C153911025 @default.
- W3031298277 hasConcept C190062978 @default.
- W3031298277 hasConcept C190283241 @default.
- W3031298277 hasConcept C29537977 @default.
- W3031298277 hasConcept C33161422 @default.
- W3031298277 hasConcept C40767141 @default.
- W3031298277 hasConcept C54009773 @default.
- W3031298277 hasConcept C54355233 @default.
- W3031298277 hasConcept C86803240 @default.
- W3031298277 hasConcept C92087593 @default.
- W3031298277 hasConcept C95444343 @default.
- W3031298277 hasConceptScore W3031298277C104317684 @default.
- W3031298277 hasConceptScore W3031298277C153911025 @default.
- W3031298277 hasConceptScore W3031298277C190062978 @default.
- W3031298277 hasConceptScore W3031298277C190283241 @default.
- W3031298277 hasConceptScore W3031298277C29537977 @default.
- W3031298277 hasConceptScore W3031298277C33161422 @default.
- W3031298277 hasConceptScore W3031298277C40767141 @default.
- W3031298277 hasConceptScore W3031298277C54009773 @default.
- W3031298277 hasConceptScore W3031298277C54355233 @default.
- W3031298277 hasConceptScore W3031298277C86803240 @default.
- W3031298277 hasConceptScore W3031298277C92087593 @default.
- W3031298277 hasConceptScore W3031298277C95444343 @default.
- W3031298277 hasIssue "11" @default.
- W3031298277 hasLocation W30312982771 @default.
- W3031298277 hasOpenAccess W3031298277 @default.
- W3031298277 hasPrimaryLocation W30312982771 @default.
- W3031298277 hasRelatedWork W141783459 @default.
- W3031298277 hasRelatedWork W2350635039 @default.
- W3031298277 hasRelatedWork W2356774747 @default.
- W3031298277 hasRelatedWork W2358068046 @default.
- W3031298277 hasRelatedWork W2359963700 @default.
- W3031298277 hasRelatedWork W2360691602 @default.
- W3031298277 hasRelatedWork W2361240596 @default.
- W3031298277 hasRelatedWork W2363297395 @default.
- W3031298277 hasRelatedWork W2364422339 @default.
- W3031298277 hasRelatedWork W2370170158 @default.
- W3031298277 hasRelatedWork W2370356497 @default.
- W3031298277 hasRelatedWork W2374730845 @default.
- W3031298277 hasRelatedWork W2380892783 @default.
- W3031298277 hasRelatedWork W2386776901 @default.
- W3031298277 hasRelatedWork W2387802211 @default.
- W3031298277 hasRelatedWork W2391687467 @default.
- W3031298277 hasRelatedWork W2392113508 @default.
- W3031298277 hasRelatedWork W2393328745 @default.
- W3031298277 hasRelatedWork W2394881241 @default.
- W3031298277 hasRelatedWork W3032832296 @default.
- W3031298277 hasVolume "18" @default.
- W3031298277 isParatext "false" @default.
- W3031298277 isRetracted "false" @default.
- W3031298277 magId "3031298277" @default.
- W3031298277 workType "article" @default.