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- W3031462010 abstract "ObjectiveTo expore multi-drug resistant Acinetobacter baumannii efflux pump phenotype and efflux pump gene expression in the resistant isolates. MethodsApplication of K-B method to detect 96 strains isolated from the First Hospital of Hebei Medical University multi-drug resistant Acinetobacter baumannii′ resistance to 15 kinds of antibacterial drugs, detecting multi-drug resistant Acinetobacter baumannii efflux pump phenotype with broth microdilution method by the addition of carbonyl cyanide chlorobenzene hydrazone (CCCP) pump inhibitors,using PCR amplification and sequencing to study efflux pump protein gene sequence characteristics . ResultsThe Acinetobacter baumannii resistance rate of 96 strains to quinolones, cephalosporins, aminoglycosides, tetracyclines were 70.8%-94.8%.There were 34 positive efflux pump phenotypes in 96 multi-drug resistant Acinetobacter baumannii strains, including 33 adeB strains, 32 adeR strains, 33 adeS strains, 33 adeJ strains,0 adeE strain,33 adeM strains, positive detection rate were 97.06%, 94.12%,97.06%,97.06%,0,97.06%,respectively. By sequence comparison, adeB, adeR and adeS genes sequence homology was 100% in the GenBank. ConclusionActive efflux pump gene perturbation is one of the important factors in multi-drug resistant Acinetobacter baumannii.(Chin J Lab Med,2014,37:763-766)Key words: Acinetobacter baumannii; Drug resistance, multiple, bacterial; Carbonyl cyanide m-chlorophenyl hydrazone; Phenotype; Genotype" @default.
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- W3031462010 date "2014-10-11" @default.
- W3031462010 modified "2023-09-27" @default.
- W3031462010 title "Relationship between efflux pump phenotype and genotype in multiple drug Acinetobacter baumannii" @default.
- W3031462010 doi "https://doi.org/10.3760/cma.j.issn.1009-9158.2014.10.011" @default.
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